Organiser et accompagner la mobilité professionnelle des populations faiblement qualifiées

Presentation (2005, January)

Kennen als een vorm van handelen. John Deweys transactionele kentheorie.

in Bijdragen Tijdschrift voor Filosofie en Theologie (2005), 15(1), 5-12

Unified European Gravity Reference Network 2002 (UEGN02)—Status 2004

in Jekeli, C.; Bastos, L.; Fernandes, J. (Eds.) Gravity, Geoid and Space Missions - GGSM 2004 (2005)

Geography, Multinationals and Local Development

in Annals of Regional Science (2005), 39

Gaussian limits for vector-valued multiple stochastic integrals

in Séminaire de Probabilités XXXVIII (2005)

Interleukin-8 primes oxidative burst in neutrophil-like HL-60 through changes in cytosolic calcium

in Cell Calcium (2005), 37(6), 531-40

In response to a variety of stimuli, neutrophils release large amount of reactive oxygen species (ROS) generated by NADPH oxidase. This process known as the respiratory burst is dependent on cytosolic ... [more ▼]

In response to a variety of stimuli, neutrophils release large amount of reactive oxygen species (ROS) generated by NADPH oxidase. This process known as the respiratory burst is dependent on cytosolic free calcium concentration ([Ca(2+)](i)). Proinflammatory cytokines such as interleukin-8 (IL-8) may modulate ROS generation through a priming phenomenon. The aim of this study was to determine the effect of human IL-8 on ROS production in neutrophil-like dimethylsulfoxide-differentiated HL-60 cells (not equalHL-60 cells) and further to examine the role of Ca(2+) mobilization during the priming. IL-8 at 10 nM induced no ROS production but a [Ca(2+)](i) rise (254 +/- 36 nM). IL-8 induced a strongly enhanced (2 fold) ROS release during stimulation with 1 microM of N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLF). This potentiation of ROS production is dependent of extracellular Ca(2+) (17.0+/-4.5 arbitrary units (A.U.) in the absence of Ca(2+) versus 56.6 +/- 3.9 A.U. in the presence of 1.25 mM of Ca(2+)). Also, IL-8 enhanced fMLF-stimulated increase in [Ca(2+)](i) (375 +/- 35 versus 245 +/- 21 nM, 0.1 microM of fMLF). IL-8 had no effect on not equalHL-60 cells in response to 1 microM of thapsigargin (472 +/- 66 versus 470 +/- 60 nM). In conclusion, Ca(2+) influx is necessary for a full induction of neutrophil priming by IL-8. [less ▲]

Une plateforme collaborative et ouverte de testing assisté par ordinateur permettant l'élaboration et la passation d'instruments d'évlauation par voie d'Internet 18e Colloque international de l'ADMEE-Europe:"Comment évaluer?Outils,dispositifs et acteurs"

in Actes du 18e colloque de l’ADMEE-Europe Reims 2005: Comment évaluer? Outils, dispositifs, acteurs. (2005)

Germanistik im Bologna-Prozess

in DAAD (Ed.) Perspektiven der Germanistik in Europa (2005)

Actin-filament cross-linking protein T-plastin increases Arp2/3-mediated actin-based movement

in Journal of Cell Science (2005), 118(6), 1255-1265

Increasing evidence suggests that actin cross-linking or bundling proteins might not only structure the cortical actin cytoskeleton but also control actin dynamics. Here, we analyse the effects of T ... [more ▼]

Increasing evidence suggests that actin cross-linking or bundling proteins might not only structure the cortical actin cytoskeleton but also control actin dynamics. Here, we analyse the effects of T-plastin/T-fimbrin, a representative member of an important actin-filament cross-linking protein by combining a quantitative biomimetic motility assay with biochemical and cell-based approaches. Beads coated with the VCA domain of the Wiskott/Aldrich-syndrome protein (WASP) recruit the actin-nucleating Arp2/3 complex, polymerize actin at their surface and undergo movement when placed in cell-free extracts. T-Plastin increased the velocity of VCA beads 1.5 times, stabilized actin comets and concomitantly displaced cofilin, an actin-depolymerizing protein. T-Plastin also decreased the F-actin disassembly rate and inhibited cofilin-mediated depolymerization of actin filaments in vitro. Importantly, a bundling-incompetent variant comprising the first actin-binding domain (ABD1) had similar effects. In cells, this domain induced the formation of long actin cables to which other actin-regulating proteins were recruited. Altogether, these results favor a mechanism in which binding of ABD1 controls actin turnover independently of cross-link formation. In vivo, this activity might contribute to the assembly and maintenance of the actin cytoskeleton of plasma-membrane protrusions. [less ▲]

Etiology of Obesity

in Munsch, S.; Beglinger, P. (Eds.) Obesity and Binge Eating Disorder (2005)