Browse ORBilu by Open Access
- What it is and what it isn't
- Green Road / Gold Road?
- Ready to Publish. Now What?
- How can I support the OA movement?
- Where can I learn more?
ORBilu is a project developed by
Showing results 1 to 20 of 69
 Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoidsSmits, Lisa  ; Magni, Stefano ; Grzyb, Kamil et alE-print/Working paper (2019) Human stem cell-derived organoids have great potential for modelling physiological and pathological processes. They recapitulate in vitro the organisation and function of a respective organ or part of an ... [more ▼] Human stem cell-derived organoids have great potential for modelling physiological and pathological processes. They recapitulate in vitro the organisation and function of a respective organ or part of an organ. Human midbrain organoids (hMOs) have been described to contain midbrain-specific dopaminergic neurons that release the neurotransmitter dopamine. However, the human midbrain contains also additional neuronal cell types, which are functionally interacting with each other. Here, we analysed hMOs at high-resolution by means of single-cell RNA-sequencing (scRNA-seq), imaging and electrophysiology to unravel cell heterogeneity. Our findings demonstrate that hMOs show essential neuronal functional properties as spontaneous electrophysiological activity of different neuronal subtypes, including dopaminergic, GABAergic, and glutamatergic neurons. Recapitulating these in vivo features makes hMOs an excellent tool for in vitro disease phenotyping and drug discovery. [less ▲] Detailed reference viewed: 163 (39 UL)  Automated microfluidic cell culture of stem cell derived dopaminergic neurons; ; et al in Scientific Reports (2019) Detailed reference viewed: 119 (8 UL)Neural Stem Cells of Parkinson's Disease Patients Exhibit Aberrant Mitochondrial Morphology and Functionality; Bolognin, Silvia ; Antony, Paul et alin Stem Cell Reports (2019) Detailed reference viewed: 122 (13 UL)Non-proteolytic ubiquitination of OTULIN regulates NF-κB signaling pathway; ; et al in Journal of Molecular Cell Biology (2019) Detailed reference viewed: 35 (0 UL)Modeling Parkinson’s disease in midbrain-like organoidsSmits, Lisa ; ; et alin NPJ Parkinson's Disease (2019) Detailed reference viewed: 131 (12 UL)Guidelines for Fluorescent Guided Biallelic HDR Targeting Selection With PiggyBac System Removal for Gene EditingJarazo, Javier ; ; Schwamborn, Jens Christian in Frontiers in Genetics (2019) Detailed reference viewed: 106 (8 UL)Automated high-throughput highcontent autophagy and mitophagy analysis platform; Jarazo, Javier ; et alin Scientific Reports (2019) Detailed reference viewed: 50 (3 UL)3D Cultures of Parkinson's Disease‐Specific Dopaminergic Neurons for High Content Phenotyping and Drug TestingBolognin, Silvia ; ; et alin Advanced Science (2018) Parkinson's disease (PD)‐specific neurons, grown in standard 2D cultures, typically only display weak endophenotypes. The cultivation of PD patient‐specific neurons, derived from induced pluripotent stem ... [more ▼] Parkinson's disease (PD)‐specific neurons, grown in standard 2D cultures, typically only display weak endophenotypes. The cultivation of PD patient‐specific neurons, derived from induced pluripotent stem cells carrying the LRRK2‐G2019S mutation, is optimized in 3D microfluidics. The automated image analysis algorithms are implemented to enable pharmacophenomics in disease‐relevant conditions. In contrast to 2D cultures, this 3D approach reveals robust endophenotypes. High‐content imaging data show decreased dopaminergic differentiation and branching complexity, altered mitochondrial morphology, and increased cell death in LRRK2‐G2019S neurons compared to isogenic lines without using stressor agents. Treatment with the LRRK2 inhibitor 2 (Inh2) rescues LRRK2‐G2019S‐dependent dopaminergic phenotypes. Strikingly, a holistic analysis of all studied features shows that the genetic background of the PD patients, and not the LRRK2‐G2019S mutation, constitutes the strongest contribution to the phenotypes. These data support the use of advanced in vitro models for future patient stratification and personalized drug development. [less ▲] Detailed reference viewed: 190 (30 UL)A complex of the ubiquitin ligase TRIM32 and the deubiquitinase USP7 balances the level of c-Myc ubiquitination and thereby determines neural stem cell fate specification; ; Okawa, Satoshi et alin Cell Death & Differentiation (2018) Detailed reference viewed: 95 (11 UL)Activity of translation regulator eukaryotic elongation factor-2 kinase is increased in Parkinson disease brain and its inhibition reduces alpha synuclein toxicity; ; et al in Acta Neuropathologica Communications (2018) Detailed reference viewed: 72 (4 UL)Millifluidic culture improves human midbrain organoid vitality and differentiationBerger, Emanuel ; ; Paczia, Nicole et alin Lab on a Chip - Miniaturisation for Chemistry and Biology (2018) Detailed reference viewed: 191 (39 UL)Is Parkinson’s disease a neurodevelopmental disorder and will brain organoids help us to understand it?Schwamborn, Jens Christian in Stem Cells & Development (2018) Detailed reference viewed: 234 (13 UL)Nuclear localization and phosphorylation modulate pathological effects of Alpha-Synuclein; ; et al in Human Molecular Genetics (2018) Detailed reference viewed: 73 (8 UL)Advanced Good Cell Culture Practice for human primary, stem cell-derived and organoid models as well as microphysiological systems; ; et al in ALTEX : Alternativen zu Tierexperimenten (2018) Detailed reference viewed: 107 (6 UL)In vivo Phenotyping of Human Parkinson’s Disease-Specific Stem Cells Carrying the LRRK2-G2019S Mutation Reveals Increased a-Synuclein Levels but Absence of Spreading; Smits, Lisa ; Bolognin, Silvia et alin Opera Medica et Physiologica (2018) Detailed reference viewed: 63 (18 UL) Automated micro uidic cell culture of stem cell derived dopaminergic neurons in Parkinson's diseaseKane, Khalid ; Lucumi Moreno, Edinson ; Hachi, Siham et alin bioRxiv (2017) Detailed reference viewed: 127 (13 UL)FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's Disease ModelingArias, Jonathan ; Jarazo, Javier ; Qing, Xiaobing et alin Stem Cell Reports (2017) Detailed reference viewed: 252 (7 UL)Human induced pluripotent stem cell-derived neuronal progenitors are a suitable and effective drug discovery model for neurological mtDNA disordersWalter, Jonas ; Nickels, Sarah Louise ; Schwamborn, Jens Christian in Stem Cell Investigation (2017) Detailed reference viewed: 84 (7 UL)CRISPR/Cas9 and piggyBac-mediated footprint-free LRRK2-G2019S knock-in reveals neuronal complexity phenotypes and α-Synuclein modulation in dopaminergic neurons; Walter, Jonas ; Jarazo, Javier et alin Stem Cell Research (2017) Detailed reference viewed: 111 (5 UL)In Vivo Phenotyping Of Parkinson-Specific Stem Cells Reveals Increased a-Synuclein Levels But No SpreadingHemmer, Kathrin ; Smits, Lisa ; Bolognin, Silvia et alin bioRxiv (2017) Detailed reference viewed: 160 (24 UL) |
||
