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See detailDetection of Differentially Modified Pathogen Proteins by Western Blot after 2D Gel Electrophoresis and Identification by MALDI-TOF/TOF
Fack, F; Kessler, Julia UL; Pirrotte, P et al

in Stulik, J; Toman, R; Butaye, P (Eds.) et al BSL3 and BSL4 agents : proteomics, glycomics, and antigenicity (2011)

The detection of proteomic changes after viral infection, especially those which are due to post-translational modifications of host and pathogen proteins is of particular importance for the understanding ... [more ▼]

The detection of proteomic changes after viral infection, especially those which are due to post-translational modifications of host and pathogen proteins is of particular importance for the understanding of the fast interplay between pathogen and host components in viral infections. The characterization of modified isoforms of such proteins can benefit considerably from the combination of fluorescence labelled monospecific antibodies and 2D-DIGE differential proteomic studies. The potential of this approach is illustrated with a study of essential proteins in a measles virus-host cell system using small 2D gels and low sample amounts. [less ▲]

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See detailHeteroduplex mobility assay (HMA) pre-screening: An improved strategy for the rapid identification of inserts selected from phage-displayed peptide libraries
Fack, F.; Deroo, S.; Kreis, Stephanie UL et al

in Molecular Diversity (2000), 5(1), 7-12

Phage-displayed peptide libraries represent an efficient tool to isolate peptides that bind a given target molecule. After several selection rounds, generally a large pool of target binding phages is ... [more ▼]

Phage-displayed peptide libraries represent an efficient tool to isolate peptides that bind a given target molecule. After several selection rounds, generally a large pool of target binding phages is obtained. Conventional analysis of the selected phage population involves extensive sequencing of many clones, most of which can be identical. We have adapted the Heteroduplex Mobility Assay (HMA) for pre-screening of phage inserts that were amplified by direct colony PCR of ELISA-positive clones. This strategy allowed for the rapid and reproducible assignment of insert sequences to different 'heteroduplex migration groups'. Sequence analysis of only one representative of each HMA migration group then completes the characterisation of the binding phage population. In our model experiments, only 16% of HMA pre-screened clones required further sequence analysis. [less ▲]

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