References of "Dannert, R."
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailDetermination of the rheological properties of Matrigel for optimum seeding conditions in microfluidic cell cultures
Kane, K. I. W.; Moreno, E. Lucumi; Lehr, Claudius Moritz UL et al

in AIP ADVANCES (2018), 8(12),

Hydrogels are increasingly used as a surrogate extracellular matrix in three-dimensional cell culture systems, including microfluidic cell culture. Matrigel is a hydrogel of natural origin widely used in ... [more ▼]

Hydrogels are increasingly used as a surrogate extracellular matrix in three-dimensional cell culture systems, including microfluidic cell culture. Matrigel is a hydrogel of natural origin widely used in cell culture, particularly in the culture of stem cell-derived cell lines. The use of Matrigel as a surrogate extracellular matrix in microfluidic systems is challenging due to its biochemical, biophysical, and biomechanical properties. Therefore, understanding and characterising these properties is a prerequisite for optimal use of Matrigel in microfluidic systems. We used rheological measurements and particle image velocimetry to characterise the fluid flow dynamics of liquefied Matrigel during loading into a three-dimensional microfluidic cell culture device. Using fluorescence microscopy and fluorescent beads for particle image velocimetry measurements (velocity profiles) in combination with classical rheological measurements of Matrigel (viscosity versus shear rate), we characterised the shear rates experienced by cells in a microfluidic device for three-dimensional cell culture. This study provides a better understanding of the mechanical stress experienced by cells, during seeding of a mixture of hydrogel and cells, into three-dimensional microfluidic cell culture devices. (C) 2018 Author(s). [less ▲]

Detailed reference viewed: 108 (3 UL)
Full Text
Peer Reviewed
See detailInfluence of suspension viscosity on Brownian relaxation of filler particles
Dannert, R.; Winter, H. H.; Sanctuary, Roland UL et al

in Rheologica Acta (2017), 56(7-8), 615-622

Brownian relaxation caused by Brownian movement of particles in suspensions can macroscopically be probed by small-amplitude oscillatory shear experiments. Phenomenological considerations suggest a direct ... [more ▼]

Brownian relaxation caused by Brownian movement of particles in suspensions can macroscopically be probed by small-amplitude oscillatory shear experiments. Phenomenological considerations suggest a direct proportionality between suspension viscosity and Brownian relaxation times. To verify this relation experimentally, a set of nanocomposite suspensions with viscosities varying over five decades is presented. The suspensions are chosen in a way to ensure that particle-particle interactions and average particle-particle distances are identical so that they can be used as a model system to study the mere influence of suspension viscosity on Brownian relaxation. The suggested linear relationship between suspension viscosity and Brownian relaxation time can be confirmed. Moreover, a verification of a recently introduced characteristic timescale for Brownian relaxation is presented. [less ▲]

Detailed reference viewed: 70 (2 UL)