References of "Bernard, Yohann 40000053"
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See detailCell passaging rapidly affects expression, secretion and activity of MMP9 as well as mobility of HL60 leukemia cells
Bernard, Yohann UL; Plançon, Sébastien UL; Melchior, Chantal UL et al

in Journal of Cell and Animal Biology (2008), 2(9), 160-165

The HL60 cell line, derived from acute promyelocytic leukemia cells, can differentiate into neutrophil-like cell following DMSO treatment. Mobility of HL60, or DMSO-differentiated HL60 cells (≠HL60 ... [more ▼]

The HL60 cell line, derived from acute promyelocytic leukemia cells, can differentiate into neutrophil-like cell following DMSO treatment. Mobility of HL60, or DMSO-differentiated HL60 cells (≠HL60), requires surface expression of adhesion molecules and production of matrix metalloproteinases (MMPs). The aim of this study was to investigate in HL60 and ≠HL60 the effects of cell passaging (over 5 passages after delivery (P and P+5)) on i) surface expression of adhesion molecule CD11b, which is considered a neutrophil differentiation marker ii) MMP9 mRNA expression, protein release and zymographic activity and iii) cellular mobility. As expected, CD11b expression at both cell passages increased in ≠HL60 relative to undifferentiated HL60, but expression levels of this neutrophils marker did not change over 5 passages. MMP9 mRNA expression however, in basal conditions was increased in HL60 at P+5. At P+5 versus P, MMP9 protein levels, MMP9 zymographic activity and cellular mobility in HL60 and ≠HL60 were elevated. Stimulation by N-formyl-L-Methionyl-L-Leucyl-L-Phenylalanine had no effects on HL60, but raised MMP9 protein concentration and zymographic activity in ≠HL60. Since passage history is likely to also influence cellular functions other than MMP-related effects, it is important to carefully consider passage numbers when designing experiments [less ▲]

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See detailCo-cultures of Human Coronary Smooth Muscle Cells and Dimethyl Sulfoxide-differentiated HL60 Cells Upregulate ProMMP9 Activity and Promote Mobility"”Modulation By Reactive Oxygen Species
Bernard, Yohann UL; Melchior, Chantal UL; Tschirhart, Eric UL et al

in Inflammation (2008)

Vascular cells and leukocytes, involved in the development of atherosclerosis, produce cytokines and/or reactive oxygen species (ROS) and matrix metalloproteinases (MMPs) implicated in cell mobility. We ... [more ▼]

Vascular cells and leukocytes, involved in the development of atherosclerosis, produce cytokines and/or reactive oxygen species (ROS) and matrix metalloproteinases (MMPs) implicated in cell mobility. We investigated by co-culture experiments the effects of human coronary smooth muscle cells (HCSMC) on MMPs characteristics and mobility of neutrophil-like dimethyl sulfoxide-differentiated HL60 cells (≠HL60). The effects of superoxide dismutase (SOD) and catalase were also analyzed. All the studied MMP2 characteristics remained unchanged. HCSMC stimulated MMP9 protein level, activity and mobility of ≠HL60 cells and expressed and secreted a variety of cytokines implicated in atherosclerosis. SOD and catalase increased MMP9 expression, protein level and activity of ≠HL60, but migration of ≠HL60 cells was only decreased by catalase, demonstrating that ROS are more efficient in modulating MMP9 activity of ≠HL60 than their mobility. Finally, HCSMC being able to stimulate ≠HL60, their co-cultures may represent an in vitro approach to study cellular interactions occurring in vivo during atherosclerosis. [less ▲]

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