References of "Whistler, T."
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See detailSequence analysis of the nucleocapsid gene of measles virus isolates from South Africa identifies a new genotype
Kreis, Stephanie UL; Vardas, E.; Whistler, T.

in Journal of General Virology (1997), 78(7), 1581-1587

Sequence analysis was performed on 20 measles virus (MV) isolates from South Africa, five of which were obtained between 1986 and 1989 and 15 isolates collected during the 1994/95 measles season. A 590 bp ... [more ▼]

Sequence analysis was performed on 20 measles virus (MV) isolates from South Africa, five of which were obtained between 1986 and 1989 and 15 isolates collected during the 1994/95 measles season. A 590 bp fragment of the carboxyl terminus of the nucleocapsid (N) was amplified by PCR and subjected to sequence and phylogenetic analysis. Comparison of the South African MV strains with those previously described revealed that at least two distinct groups of wild-type (wt) MV exist, one of which has been circulating since 1986. The major genotype (I) was represented by the more recent isolates which showed three characteristic amino acid substitutions. Furthermore, three vaccine-like viruses with sequences very similar to the Edmonston wt strain were identified. Phylogenetic analysis of 100 MV strains allowed the assignment of new definitions for MV genotypes and subgroups. Employing these definitions, the majority of South African isolates analysed here formed a new genotype. [less ▲]

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Peer Reviewed
See detailRapid identification of measles virus strains by the heteroduplex mobility assay
Kreis, Stephanie UL; Whistler, T.

in Virus Research (1997), 47(2), 472197-203

The continued endemic presence of measles virus (MV), and the large number of isolates which are made in South Africa each year, demanded the use of a rapid and reliable pre-screening technique to select ... [more ▼]

The continued endemic presence of measles virus (MV), and the large number of isolates which are made in South Africa each year, demanded the use of a rapid and reliable pre-screening technique to select isolates for molecular epidemiological studies by sequence analysis. The heteroduplex mobility assay (HMA) was used to genetically characterize 47 MV isolates collected from three different provinces in South Africa, made between 1986 and 1995. The carboxyl-terminal 590 nt of the nucleocapsid (N) gene - the most variable region of the genome - was amplified by polymerase chain reaction (PCR) and subsequently subjected to HMA analysis for initial genotyping. The results showed three different patterns of heteroduplex formation by gel electrophoresis, representing two distinct wild-type lineages and one group of vaccine-like viruses. Comparison of HMA results with phylogenetic analysis of sequence data for several of the South African MV strains showed a complete correlation of results. The HMA proved to be a useful tool for screening MV isolates for use in molecular epidemiological studies. [less ▲]

Detailed reference viewed: 81 (18 UL)