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See detailDie Emanzipation des hybriden Selbst: Identität, Kultur und Literatur in Luxemburg
Baumann, Isabell UL

Book published by transcipt (in press)

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See detailIntegrated Analyses of Microbiome and Longitudinal Metabolome Data Reveal Microbial-Host Interactions on Sulfur Metabolism in Parkinson’s Disease
Hertel, Johannes; Harms, Amy C.; Heinken, Almut et al

in Cell Reports (in press)

Parkinson’s disease (PD) exhibits systemic effects on human metabolism with emerging roles for the gut microbiome. Here, we integrated longitudinal metabolome data from 30 drug-naïve, de-novo PD patients ... [more ▼]

Parkinson’s disease (PD) exhibits systemic effects on human metabolism with emerging roles for the gut microbiome. Here, we integrated longitudinal metabolome data from 30 drug-naïve, de-novo PD patients and 30 matched controls with constraint-based modeling of gut microbial communities derived from an independent, drug-naïve PD cohort, and prospective data from a general population. Our key results are i) longitudinal trajectory of metabolites associated with the interconversion of methionine and cysteine via cystathionine differed between PD patients and controls, ii) dopaminergic medication showed strong lipidomic signatures, iii) taurine-conjugated bile acids correlated with the severity of motor symptoms, while low levels of sulfated taurolithocholate were associated with incident PD in the general population, and iv) computational modeling predicted changes in sulfur metabolism, driven by A. muciniphila and B. wadsworthia, consistent with the changed metabolome. In conclusion, the multi-omics integration revealed PD-specific patterns in microbial-host sulfur co-metabolism that may contribute to PD severity. [less ▲]

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See detailThe Power of LC-MS Based Multiomics: Exploring Adipogenic Differentiation of Human Mesenchymal Stem/Stromal Cells
Rampler, Evelyn; Egger, Dominik; Schoeny, Harald et al

in Molecules (2019)

The molecular study of fat cell development in the human body is essential for our understanding of obesity and related diseases. Mesenchymal stem/stromal cells (MSC) are the ideal source to study fat ... [more ▼]

The molecular study of fat cell development in the human body is essential for our understanding of obesity and related diseases. Mesenchymal stem/stromal cells (MSC) are the ideal source to study fat formation as they are the progenitors of adipocytes. In this work, we used human MSCs, received from surgery waste, and differentiated them into fat adipocytes. The combination of several layers of information coming from lipidomics, metabolomics and proteomics enabled network analysis of the biochemical pathways in adipogenesis. Simultaneous analysis of metabolites, lipids, and proteins in cell culture is challenging due to the compound’s chemical difference, so most studies involve separate analysis with unimolecular strategies. In this study, we employed a multimolecular approach using a two–phase extraction to monitor the crosstalk between lipid metabolism and protein-based signaling in a single sample (~105 cells). We developed an innovative analytical workflow including standardization with in-house produced 13C isotopically labeled compounds, hyphenated high-end mass spectrometry (high-resolution Orbitrap MS), and chromatography (HILIC, RP) for simultaneous untargeted screening and targeted quantification. Metabolite and lipid concentrations ranged over three to four orders of magnitude and were detected down to the low fmol (absolute on column) level. Biological validation and data interpretation of the multiomics workflow was performed based on proteomics network reconstruction, metabolic modelling (MetaboAnalyst 4.0), and pathway analysis (OmicsNet). Comparing MSCs and adipocytes, we observed significant regulation of different metabolites and lipids such as triglycerides, gangliosides, and carnitine with 113 fully reprogrammed pathways. The observed changes are in accordance with literature findings dealing with adipogenic differentiation of MSC. These results are a proof of principle for the power of multimolecular extraction combined with orthogonal LC-MS assays and network construction. Considering the analytical and biological validation performed in this study, we conclude that the proposed multiomics workflow is ideally suited for comprehensive follow-up studies on adipogenesis and is fit for purpose for different applications with a high potential to understand the complex pathophysiology of diseases. [less ▲]

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See detailParadigmenwechsel in der Lehrerausbild
Baumann, Isabell UL; Harion, Dominic UL

Article for general public (2019)

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See detailARCHITECTURES AND SYNCHRONIZATION TECHNIQUES FOR COHERENT DISTRIBUTED REMOTE SENSING SYSTEMS
Merlano Duncan, Juan Carlos UL; Querol Borras, Jorge UL; Camps, Adriano et al

in 2019 IEEE International Geoscience and Remote Sensing Symposium (IGARSS) (2019, August 31)

Phase, frequency and time synchronization is a crucial requirement for many applications as such as multi-static remote sensing and distributed beamforming for communications. The literature on the field ... [more ▼]

Phase, frequency and time synchronization is a crucial requirement for many applications as such as multi-static remote sensing and distributed beamforming for communications. The literature on the field is very wide, and in some cases, the requirements of the proposed synchronization solution may surpass the ones set by the application itself. Moreover, the synchronization solution becomes even more challenging when the nodes are flying or hovering on aerial or space platforms. In this work, we compare and classify the synchronization technologies available in the literature according to a common proposed framework, and we discuss the considerations of an implementation for distributed remote sensing applications. The general framework considered is based on a distributed collection of autonomous nodes that try to synchronize their clocks with a common reference. Moreover, they can be classified in non-overlapping, adjacent and overlapping frequency band scenarios [less ▲]

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See detailEducational expansion and inequalities: how did inequalities by social origin and gender decrease in modern industrial societies
Hadjar, Andreas UL

in Becker, Rolf (Ed.) Research Handbook on the Sociology of Education (2019)

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