Reference : A biomolecular isolation framework for eco-systems biology
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Biotechnology
Life sciences : Environmental sciences & ecology
Life sciences : Microbiology
http://hdl.handle.net/10993/912
A biomolecular isolation framework for eco-systems biology
English
Roume, Hugo [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Muller, Emilie [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Cordes, Thekla [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Renaut, Jenny [> >]
Hiller, Karsten [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Wilmes, Paul mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
2013
ISME Journal (The)
7
1
110-121
Yes
International
1751-7362
1751-7370
[en] biomolecules ; eco-systems biology ; extraction ; isolation ; metabolomics ; sample heterogeneity
[en] Mixed microbial communities are complex, dynamic and heterogeneous. It is therefore essential that biomolecular fractions obtained for high-throughput omic analyses are representative of single samples to facilitate meaningful data integration, analysis and modeling. We have developed a new methodological framework for the reproducible isolation of high-quality genomic DNA, large and small RNA, proteins, and polar and non-polar metabolites from single unique mixed microbial community samples. The methodology is based around reproducible cryogenic sample preservation and cell lysis. Metabolites are extracted first using organic solvents, followed by the sequential isolation of nucleic acids and proteins using chromatographic spin columns. The methodology was validated by comparison to traditional dedicated and simultaneous biomolecular isolation methods. To prove the broad applicability of the methodology, we applied it to microbial consortia of biotechnological, environmental and biomedical research interest. The developed methodological framework lays the foundation for standardized molecular eco-systematic studies on a range of different microbial communities in the future.
Luxembourg Centre for Systems Biomedicine (LCSB): Eco-Systems Biology (Wilmes Group) ; Luxembourg Centre for Systems Biomedicine (LCSB): Metabolomics (Hiller Group)
http://hdl.handle.net/10993/912
10.1038/ismej.2012.72
Paid Open Access

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