Reference : Correlation between growth rates, EIIACrr phosphorylation, and intracellular cyclic A...
Scientific journals : Article
Life sciences : Multidisciplinary, general & others
http://hdl.handle.net/10993/805
Correlation between growth rates, EIIACrr phosphorylation, and intracellular cyclic AMP levels in Escherichia coli K-12.
English
Bettenbrock, Katja [> >]
Sauter, Thomas mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Jahreis, Knut [> >]
Kremling, Andreas [> >]
Lengeler, Joseph W. [> >]
Gilles, Ernst-Dieter [> >]
2007
Journal of Bacteriology
189
19
6891-900
Yes (verified by ORBilu)
0021-9193
1098-5530
United States
[en] Bioreactors/microbiology ; Cyclic AMP/metabolism ; Cyclic AMP Receptor Protein/genetics/metabolism ; Escherichia coli/genetics/growth & development/metabolism ; Escherichia coli Proteins/genetics/metabolism ; Gene Expression Regulation, Bacterial ; Glucose/metabolism ; Mannitol/metabolism ; Phosphoenolpyruvate Sugar Phosphotransferase System/genetics/metabolism ; Phosphorylation ; Promoter Regions, Genetic/genetics ; Sucrose/metabolism
[en] In Escherichia coli K-12, components of the phosphoenolpyruvate-dependent phosphotransferase systems (PTSs) represent a signal transduction system involved in the global control of carbon catabolism through inducer exclusion mediated by phosphoenolpyruvate-dependent protein kinase enzyme IIA(Crr) (EIIA(Crr)) (= EIIA(Glc)) and catabolite repression mediated by the global regulator cyclic AMP (cAMP)-cAMP receptor protein (CRP). We measured in a systematic way the relation between cellular growth rates and the key parameters of catabolite repression, i.e., the phosphorylated EIIA(Crr) (EIIA(Crr) approximately P) level and the cAMP level, using in vitro and in vivo assays. Different growth rates were obtained by using either various carbon sources or by growing the cells with limited concentrations of glucose, sucrose, and mannitol in continuous bioreactor experiments. The ratio of EIIA(Crr) to EIIA(Crr) approximately P and the intracellular cAMP concentrations, deduced from the activity of a cAMP-CRP-dependent promoter, correlated well with specific growth rates between 0.3 h(-1) and 0.7 h(-1), corresponding to generation times of about 138 and 60 min, respectively. Below and above this range, these parameters were increasingly uncoupled from the growth rate, which perhaps indicates an increasing role executed by other global control systems, in particular the stringent-relaxed response system.
http://hdl.handle.net/10993/805

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