Reference : Correlative microscopy for phylogenetic and ultrastructural characterization of micro...
Scientific journals : Article
Life sciences : Environmental sciences & ecology
Correlative microscopy for phylogenetic and ultrastructural characterization of microbial communities
Knierim, B. [> >]
Luef, B. [> >]
Wilmes, Paul mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Webb, R. I. [> >]
Auer, Manfred [> >]
Comolli, L. R. [> >]
Banfield, J. F. [> >]
Environmental Microbiology Reports
Yes (verified by ORBilu)
United Kingdom
[en] Transmission electron microscopy (TEM) can provide ultrastructural information for cells in microbial community samples and phylogenetic information can be recovered via molecular surveys. Here we report an approach to link these data sets by coupling fluorescence in situ hybridization (FISH) with either conventional biological or cryogenic TEM. The method could fundamentally improve our understanding of the organization and functioning of microbial communities in natural systems.

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