Reference : An alpaca single-domain antibody blocks filopodia formation by obstructing L-plastin-...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/7448
An alpaca single-domain antibody blocks filopodia formation by obstructing L-plastin-mediated F-actin bundling.
English
Delanote, Veerle [> >]
Vanloo, Berlinda [> >]
Catillon, Marie mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Friederich, Evelyne mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Vandekerckhove, Joel [> >]
Gettemans, Jan [> >]
2010
FASEB Journal
24
1
105-18
Yes (verified by ORBilu)
International
0892-6638
1530-6860
United States
[en] Actins/metabolism ; Animals ; Antibodies/chemistry/genetics/metabolism ; Antibody Affinity ; Camelids, New World/genetics/immunology ; Cell Line, Tumor ; Cell Movement/immunology/physiology ; Humans ; Immunoglobulin Heavy Chains/chemistry/genetics/metabolism ; Immunoglobulin Variable Region/chemistry/genetics/metabolism ; Male ; Microfilament Proteins/antagonists & inhibitors/genetics/immunology/metabolism ; Neoplasm Invasiveness/immunology/physiopathology ; Protein Binding ; Protein Conformation ; Protein Structure, Tertiary ; Pseudopodia/immunology/metabolism ; RNA Interference ; Recombinant Proteins/chemistry/genetics/metabolism ; Thermodynamics
[en] L-plastin, a conserved modular F-actin bundling protein, is ectopically expressed in tumor cells and contributes to cell malignancy and invasion. The underlying molecular mechanisms involved remain unclear, in part, because specific inhibitors of L-plastin are lacking. We used recombinant alpaca-derived L-plastin single-domain antibodies (nanobodies) as effector of L-plastin function in cells.
http://hdl.handle.net/10993/7448

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