Reference : Targeting of Listeria monocytogenes ActA protein to the plasma membrane as a tool to ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/6287
Targeting of Listeria monocytogenes ActA protein to the plasma membrane as a tool to dissect both actin-based cell morphogenesis and ActA function.
English
Friederich, Evelyne mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Gouin, E. [> >]
Hellio, R. [> >]
Kocks, C. [> >]
Cossart, P. [> >]
Louvard, D. [> >]
1995
The EMBO journal
14
12
2731-44
Yes (verified by ORBilu)
International
0261-4189
ENGLAND
[en] Actins/biosynthesis/metabolism ; Amino Acid Sequence ; Animals ; Bacterial Proteins/genetics/metabolism ; Base Sequence ; Cell Line ; Cell Membrane/metabolism ; Cell Size ; Cytoskeleton/metabolism ; Fibroblasts ; Haplorhini ; HeLa Cells ; Humans ; Listeria monocytogenes/metabolism ; Membrane Proteins/genetics/metabolism ; Molecular Sequence Data ; Morphogenesis ; Mutation/physiology ; Protein Sorting Signals/metabolism ; Recombinant Fusion Proteins/biosynthesis/metabolism
[en] Actin assembly on the surface of Listeria monocytogenes in the cytoplasm of infected cells provides a model to study actin-based motility and changes in cell shape. We have shown previously that the ActA protein, exposed on the bacterial surface, is required for polarized nucleation of actin filaments. To investigate whether plasma membrane-associated ActA can control the organization of microfilaments and cell shape, variants of ActA, in which the bacterial membrane signal had been replaced by a plasma membrane anchor sequence, were produced in mammalian cells. While both cytoplasmic and membrane-bound forms of ActA increased the F-actin content, only membrane-associated ActA caused the formation of plasma membrane extensions. This finding suggests that ActA acts as an actin filament nucleator and shows that permanent association with the inner face of the plasma membrane is required for changes in cell shape. Based on the observation that the amino-terminal segment of ActA and the remaining portion which includes the proline-rich repeats cause distinct phenotypic modifications in transfected cells, we propose a model in which two functional domains of ActA cooperate in the nucleation and dynamic turnover of actin filaments. The present approach is a new model system to dissect the mechanism of action of ActA and to further investigate interactions of the plasma membrane and the actin cytoskeleton during dynamic changes of cell shape.
http://hdl.handle.net/10993/6287

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