Reference : Growing an actin gel on spherical surfaces.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/6281
Growing an actin gel on spherical surfaces.
English
Noireaux, V. [> >]
Golsteyn, R. M. [> >]
Friederich, Evelyne mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Prost, J. [> >]
Antony, C. [> >]
Louvard, D. [> >]
Sykes, C. [> >]
2000
Biophysical journal
78
3
1643-54
Yes (verified by ORBilu)
International
0006-3495
UNITED STATES
[en] Actins/chemistry/physiology/ultrastructure ; Animals ; Bacterial Proteins/chemistry/physiology/ultrastructure ; Cell-Free System ; Cytosol/physiology ; Gels ; Glutathione Transferase/chemistry ; HeLa Cells ; Humans ; Listeria monocytogenes/physiology/ultrastructure ; Membrane Proteins/chemistry/physiology/ultrastructure ; Microscopy, Electron ; Microscopy, Immunoelectron ; Models, Biological ; Movement ; Muscle, Skeletal ; Rabbits ; Recombinant Fusion Proteins/chemistry/ultrastructure ; Surface Properties
[en] Inspired by the motility of the bacteria Listeria monocytogenes, we have experimentally studied the growth of an actin gel around spherical beads grafted with ActA, a protein known to be the promoter of bacteria movement. On ActA-grafted beads F-actin is formed in a spherical manner, whereas on the bacteria a "comet-like" tail of F-actin is produced. We show experimentally that the stationary thickness of the gel depends on the radius of the beads. Moreover, the actin gel is not formed if the ActA surface density is too low. To interpret our results, we propose a theoretical model to explain how the mechanical stress (due to spherical geometry) limits the growth of the actin gel. Our model also takes into account treadmilling of actin. We deduce from our work that the force exerted by the actin gel on the bacteria is of the order of 10 pN. Finally, we estimate from our theoretical model possible conditions for developing actin comet tails.
http://hdl.handle.net/10993/6281

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