Reference : Long term association of the cytokine receptor gp130 and the Janus kinase Jak1 reveal...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/6233
Long term association of the cytokine receptor gp130 and the Janus kinase Jak1 revealed by FRAP analysis
English
Giese, B. [> >]
Au-Yeung, C. K. [> >]
Herrmann, A. [> >]
Diefenbach, S. [> >]
Haan, Claude mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Küster, A. [> >]
Wortmann, S. B. [> >]
Roderburg, C. [> >]
Heinrich, P. C. [> >]
Behrmann, Iris mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Müller-Newen, G. [> >]
2003
Journal of Biological Chemistry
American Society for Biochemistry and Molecular Biology
278
40
39205-13
Yes (verified by ORBilu)
International
0021-9258
1083-351X
Baltimore
MD
[en] Animals ; Membrane Glycoproteins ; Microscopy, Confocal ; Protein Binding ; Protein-Tyrosine Kinases ; Recombinant Fusion Proteins ; Signal Transduction ; Temperature ; Time Factors ; Janus Kinase 1 ; Genetic Vectors ; Antibodies, Monoclonal ; Antigens, CD ; COS Cells ; Cell Line ; Cell Membrane ; Cytokine Receptor gp130 ; Cytoplasm ; Fluorescence Recovery After Photobleaching ; Transfection
[en] Signal transduction through cytokine receptors is mediated mainly by non-covalently associated Jak tyrosine kinases. By confocal microscopy, the cytokine receptor gp130 and Jak1, fused with either yellow (YFP) or cyan (CFP) fluorescent protein, were found to be colocalized predominantly at intracellular vesicular structures and at the plasma membrane. Quantitative fluorescence recovery after photobleaching (FRAP) analysis at the plasma membrane revealed equal mobilities for gp130-YFP and Jak1-YFP. Thus, Jak1-YFP diffuses like a transmembrane protein indicating that membrane-bound Jak1 does not exchange rapidly with cytosolic Jaks. Applying a novel dual-color FRAP approach we found that immobilization of gp130-CFP by a pair of monoclonal antibodies led to a corresponding immobilization of co-transfected Jak1-YFP. We conclude from these findings that Jak1, once bound to a gp130 molecule, does not exchange between different receptors at the plasma membrane neither via the cytoplasmic compartment nor via a membrane-associated state.
http://hdl.handle.net/10993/6233
10.1074/jbc.M303347200

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