Reference : Human umbilical cord blood-derived eosinophils cultured in the presence of IL-3 and I...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/5728
Human umbilical cord blood-derived eosinophils cultured in the presence of IL-3 and IL-5 respond to fMLP with [Ca2+]i variation and O2- production
English
Zardini, D. M. [> >]
Heuschling, Paul mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC)]
Gallois, A. [> >]
Bueb, Jean-Luc mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Tschirhart, Eric mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
1997
Journal of Immunological Methods
Elsevier Science
205
1
1-9
Yes (verified by ORBilu)
0022-1759
[en] pharmacology ; Calcium ; Cell Differentiation ; Cells, Cultured ; Eosinophils ; Female ; Fetal Blood ; Humans ; Interleukin-3 ; Interleukin-5 ; N-Formylmethionine Leucyl-Phenylalanine ; Pregnancy ; Reactive Oxygen Species ; metabolism ; cytology ; Adult
[en] In the presence of interleukin-3 and interleukin-5, eosinophil precursors from human umbilical cord blood mononuclear cells were regularly differentiated into mature eosinophil-like cells expressing normal morphology and cyanide-resistant peroxidase. O2- production and [Ca2+]i rise were measured in these in vitro differentiated eosinophils after fMLP stimulation; with dihydrorhodamine-123 and fura-2, respectively. Umbilical cord blood-derived eosinophils responded to fMLP (0.01 nM to 3 microM) with a concentration-dependent production of O2- (EC50 = 63.1 +/- 17.2 nM; Emax = 71.0 +/- 6.2 pmol/min/10(6) cells). O2- production was correlated with an fMLP concentration-dependent increase in [Ca2+]i (EC50 = 32.5 +/- 14.9 nM; Emax = 200.0 +/- 23.9 nM). These results indicate that human umbilical cord blood-derived eosinophils demonstrate functional characteristics similar to adult human peripheral blood eosinophils after activation by fMLP. Therefore, the large numbers of eosinophils (2-3 x 10(6)/ml cord blood) which can be obtained by culture of human cord blood mononuclear cells may serve as a useful model for future studies which will provide insight into the pathogenesis of diseases associated with eosinophils.
http://hdl.handle.net/10993/5728

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