Reference : Receptor-independent effects of natural cannabinoids in rat peritoneal mast cells in vitro
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/5722
Receptor-independent effects of natural cannabinoids in rat peritoneal mast cells in vitro
English
Bueb, Jean-Luc mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Lambert, D. M. [> >]
Tschirhart, Eric mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
2001
Biochimica et Biophysica Acta
1538
2-3
252-9
Yes (verified by ORBilu)
0006-3002
[en] antagonists & inhibitors ; Naphthalenes ; Morpholines ; Mast Cells ; Male ; Histamine Release ; Endocannabinoids ; Cells, Cultured ; Cannabinoids ; Benzoxazines ; Benzalkonium Compounds ; Arachidonic Acids ; Palmitic Acids ; Peritoneum ; Polyunsaturated Alkamides ; drug effects ; cytology ; pharmacology ; immunology ; drug effects ; chemical synthesis ; pharmacology ; p-Methoxy-N-methylphenethylamine ; Tetrahydrocannabinol ; Rats, Wistar ; Rats ; Animals
[en] Cannabinoids can activate CB(1) and CB(2) receptors. Since a CB(2) mRNA has been described in rat peritoneal mast cells (RPMC), we investigated a series of cannabinoids and derivatives for their capacity to stimulate RPMC. Effects of natural cannabinoids Delta(9)-tetrahydrocannabinol (Delta(9)-THC), Delta(8)-THC, endocannabinoids (anandamide, palmitoylethanolamide) and related compounds (N-decanoyl-, N-lauroyl-, N-myristoyl-, N-stearoyl- and N-oleoyl-ethanolamines; N-palmitoyl derivatives (-butylamine, -cyclohexylamine, -isopropylamine); and N-palmitoyl, O-palmitoylethanolamine), and synthetic cannabinoids including WIN 55,212-2, SR141716A and SR144528 were assessed for their capacity to induce histamine release or prime RPMC stimulated by compound 48/80. Only Delta(9)-THC and Delta(8)-THC could induce non-lytic, energy- and concentration-dependent histamine releases from RPMC (respective EC(50) values: 23.5+/-1.2; 53.4+/-20.6 microM, and maxima: 71.2+/-5.5; 55.7+/-2.7% of the total RPMC histamine content). These were not blocked by CB(1) (SR141716A) or CB(2) (SR144528) antagonists, but reduced by pertussis toxin (100 ng/ml). Endocannabinoids and analogues did neither induce histamine secretion, nor prime secretion induced by compound 48/80 (0.2 microg/ml). Delta(9)-THC and Delta(8)-THC induced in vitro histamine secretion from RPMC through CB receptor-independent interactions, partly involving G(i/o) protein activation.
http://hdl.handle.net/10993/5722

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