Reference : Promoter characterization and genomic organization of the gene encoding integrin-link...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/4925
Promoter characterization and genomic organization of the gene encoding integrin-linked kinase 1
English
Melchior, C. A [Laboratoire Franco-Luxembourgeois de Recherche Biomédicale (CNRS/CRP-Santé), University Center, 162A, avenue de la Faiencerie L-1511, Luxembourg]
Kreis, Stephanie mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Janji, B. B [> >]
Kieffer, N. A [> >]
2002
Biochimica et Biophysica Acta-Gene Structure and Expression
Elsevier Science
1575
1-3
117-122
Yes (verified by ORBilu)
0167-4781
Amsterdam
[en] Genomic sequence; Integrin; Integrin-linked kinase; Promoter activity
[en] Integrin-linked kinase (ILK)-1 is a 59-kDa serine-threonine protein kinase, which associates with the cytoplasmic domain of β1, β2 and β3 integrins and acts as a receptor proximal kinase regulating integrin-mediated signal transduction. We have recently identified an isoform of ILK (ILK-2), which is expressed, in a TGF-β1-dependent manner, in a highly invasive tumor cell line but not in normal adult tissues. In contrast, ILK-1 is ubiquitously expressed in normal tissues and is up-regulated in various tumors independent of TGF-β1. Here, we report the structural organization and the promoter activity of the human ILK-1 gene, contained within a 8.8-kb genomic fragment cloned from a human BAC library. The mature protein is encoded by 13 exons. The last coding exon contains the entire 3′ UTR of the ILK-1 gene, which overlaps with the complementary 3′ UTR sequence of the TAF2H gene, a TATA box binding protein-associated factor. A major transcriptional initiation start site was found 138 bp upstream of exon 1 in close proximity to a consensus initiator element (Inr). The ILK gene is transcribed by a TATA-less and CAAT-less promoter with typical features of housekeeping genes. The promoter activity was characterized by a luciferase reporter assay and the minimal sequence conferring promoter activity was 349 bp in size and located immediately upstream of exon 1. © 2002 Elsevier Science B.V. All rights reserved.
http://hdl.handle.net/10993/4925
10.1016/S0167-4781(02)00247-6
http://www.scopus.com/inward/record.url?eid=2-s2.0-0037012729&partnerID=40&md5=280e4ee59a89c12455190d8ffc2444ab
cited By (since 1996)11
Scopus

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