Reference : New insights into Chlamydomonas reinhardtii hydrogen production processes by combined...
Scientific journals : Article
Life sciences : Biotechnology
http://hdl.handle.net/10993/460
New insights into Chlamydomonas reinhardtii hydrogen production processes by combined microarray/RNA-seq transcriptomics.
English
Toepel, Jorg [> >]
Illmer-Kephalides, Maike [> >]
Jaenicke, Sebastian [> >]
Straube, Jasmin [> >]
May, Patrick mailto [Max-Planck-Institute for Molecular Plant Physiology - MPIMP]
Goesmann, Alexander [> >]
Kruse, Olaf [> >]
2013
Plant Biotechnology Journal
Blackwell Publishing
Yes (verified by ORBilu)
International
1467-7644
1467-7652
Oxford
United Kingdom
[en] Hydrogen production with Chlamydomonas reinhardtii induced by sulphur starvation is a multiphase process while the cell internal metabolism is completely remodelled. The first cellular response is characterized by induction of genes with regulatory functions, followed by a total remodelling of the metabolism to provide reduction equivalents for cellular processes. We were able to characterize all major processes that provide energy and reduction equivalents during hydrogen production. Furthermore, C. reinhardtii showed a strong transcript increase for gene models responsible for stress response and detoxification of oxygen radicals. Finally, we were able to determine potential bottlenecks and target genes for manipulation to increase hydrogen production or to prolong the hydrogen production phase. The investigation of transcriptomic changes during the time course of hydrogen production in C. reinhardtii with microarrays and RNA-seq revealed new insights into the regulation and remodelling of the cell internal metabolism. Both methods showed a good correlation. The microarray platform can be used as a reliable standard tool for routine gene expression analysis. RNA-seq additionally allowed a detailed time-dependent study of gene expression and determination of new genes involved in the hydrogen production process.
http://hdl.handle.net/10993/460
10.1111/pbi.12062
(c) 2013 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Limited access
pbi_12062.pdfAuthor preprint1.05 MBRequest a copy

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.