Reference : Integrated In Vitro and In Silico Modeling Delineates the Molecular Effects of a Synb...
Scientific journals : Article
Life sciences : Multidisciplinary, general & others
http://hdl.handle.net/10993/39519
Integrated In Vitro and In Silico Modeling Delineates the Molecular Effects of a Synbiotic Regimen on Colorectal-Cancer-Derived Cells
English
Greenhalgh, Kacy mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Ramiro Garcia, Javier mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Heinken []
Ullmann, Pit mailto []
Bintener, Tamara Jean Rita mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Pacheco, Maria mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Baginska, joanna mailto []
Shah, Pranjul mailto [University of Luxembourg > Rectorate > Academic Affairs >]
Frachet Bour, Audrey mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Halder, Rashi mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Fritz, Joëlle mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Sauter, Thomas mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Thiele, Ines mailto [National University of Galway]
Haan, Serge mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Letellier, Elisabeth mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Wilmes, Paul mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
30-Apr-2019
Cell Reports
Elsevier
27
1621–1632
Yes
International
2211-1247
2211-1247
Amsterdam
Netherlands
[en] By modulating the human gut microbiome, prebiotics and probiotics (combinations of which are called synbiotics) may be used to treat diseases such as colorectal cancer (CRC). Methodological limitations have prevented determining the potential combina- torial mechanisms of action of such regimens. We expanded our HuMiX gut-on-a-chip model to co-culture CRC-derived epithelial cells with a model probiotic under a simulated prebiotic regimen, and we integrated the multi-omic results with in silico metabolic modeling. In contrast to individual prebi- otic or probiotic treatments, the synbiotic regimen caused downregulation of genes involved in procarci- nogenic pathways and drug resistance, and reduced levels of the oncometabolite lactate. Distinct ratios of organic and short-chain fatty acids were produced during the simulated regimens. Treatment of primary CRC-derived cells with a molecular cocktail reflecting the synbiotic regimen attenuated self-renewal ca- pacity. Our integrated approach demonstrates the potential of modeling for rationally formulating synbi- otics-based treatments in the future.
Luxembourg Centre for Systems Biomedicine (LCSB)
Fonds National de la Recherche - FnR
Researchers ; Professionals ; Students ; General public
http://hdl.handle.net/10993/39519
10.1016/j.celrep.2019.04.001
https://www.cell.com/cell-reports/pdf/S2211-1247(19)30456-5.pdf
FnR ; FNR9964547 > Kacy Greenhalgh > > A study of the molecular mechanisms underlying the response of human colorectal adenocarcinoma enterocytes to prebiotics/probiotics > 01/03/2015 > 31/01/2019 > 2015

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