Reference : Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-sp...
E-prints/Working papers : Already available on another site
Life sciences : Biochemistry, biophysics & molecular biology
Systems Biomedicine
http://hdl.handle.net/10993/39342
Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids
English
Smits, Lisa mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)]
Magni, Stefano mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Grzyb, Kamil mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Antony, Paul mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Krüger, Rejko mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Skupin, Alexander mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Bolognin, Silvia mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Schwamborn, Jens Christian mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
28-Mar-2019
No
[en] Human stem cell-derived organoids have great potential for modelling physiological and pathological processes. They recapitulate in vitro the organisation and function of a respective organ or part of an organ. Human midbrain organoids (hMOs) have been described to contain midbrain-specific dopaminergic neurons that release the neurotransmitter dopamine. However, the human midbrain contains also additional neuronal cell types, which are functionally interacting with each other. Here, we analysed hMOs at high-resolution by means of single-cell RNA-sequencing (scRNA-seq), imaging and electrophysiology to unravel cell heterogeneity. Our findings demonstrate that hMOs show essential neuronal functional properties as spontaneous electrophysiological activity of different neuronal subtypes, including dopaminergic, GABAergic, and glutamatergic neurons. Recapitulating these in vivo features makes hMOs an excellent tool for in vitro disease phenotyping and drug discovery.
http://hdl.handle.net/10993/39342
10.1101/589598
https://www.biorxiv.org/content/10.1101/589598v1

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