Reference : Single-cell transcriptomics reveals distinct inflammation-induced microglia signatures
Scientific journals : Article
Life sciences : Multidisciplinary, general & others
http://hdl.handle.net/10993/36952
Single-cell transcriptomics reveals distinct inflammation-induced microglia signatures
English
Sousa, Carole mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Golebiewska, Anna mailto [Luxembourg Institute of Health - LIH]
Poovathingal, Suresh K mailto [Vlaams Instituut voor Biotechnologie-KU Leuven]
Kaoma, Tony mailto [Luxembourg Institute of Health - LIH]
Pires-Afonso, Yolanda mailto [University of Luxembourg]
Martina, Silvia mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)]
Coowar, Djalil mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Azuaje, Francisco mailto [Luxembourg Institute of Health - LIH]
Skupin, Alexander mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Balling, Rudi mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Biber, Knut mailto [University of Freiburg > > > ; University Medical Center Groningen, University of Groningen]
Niclou, Simone P mailto [Luxembourg Institute of Health - LIH > > > ; University of Bergen]
Michelucci, Alessandro mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
11-Sep-2018
EMBO Reports
Wiley-Blackwell
Yes (verified by ORBilu)
International
1469-221X
1469-3178
London
United States
[en] heterogeneity; ; lipopolysaccharide ; microglia; ; neuroinflammation ; single-cell RNA-seq
[en] Microglia are specialized parenchymal‐resident phagocytes of the central nervous system (CNS) that actively support, defend and modulate the neural environment. Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory processes remains largely obscure. Here, using a combination of single‐cell RNA sequencing and multicolour flow cytometry, we comprehensively profile microglia in the brain of lipopolysaccharide (LPS)‐injected mice. By excluding the contribution of other immune CNS‐resident and peripheral cells, we show that microglia isolated from LPS‐injected mice display a global downregulation of their homeostatic signature together with an upregulation of inflammatory genes. Notably, we identify distinct microglial activated profiles under inflammatory conditions, which greatly differ from neurodegenerative disease‐associated profiles. These results provide insights into microglial heterogeneity and establish a resource for the identification of specific phenotypes in CNS disorders, such as neuroinflammatory and neurodegenerative diseases.
http://hdl.handle.net/10993/36952
10.15252/embr.201846171
https://onlinelibrary.wiley.com/doi/10.15252/embr.201846171

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