Reference : Revealing new measles virus transmission routes by use of sequence analysis of phosph...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/3298
Revealing new measles virus transmission routes by use of sequence analysis of phosphoprotein and hemagglutinin genes.
English
Kessler, Julia mailto []
Kremer, Jacques R. [> >]
Shulga, Sergey V. [> >]
Tikhonova, Nina T. [> >]
Santibanez, Sabine [> >]
Mankertz, Annette [> >]
Semeiko, Galina V. [> >]
Samoilovich, Elena O. [> >]
Tamfum, Jean-Jacques Muyembe [> >]
Pukuta, Elisabeth [> >]
Muller, Claude P. [> >]
2011
Journal of Clinical Microbiology
49
2
677-83
Yes (verified by ORBilu)
0095-1137
1098-660X
United States
[en] Africa/epidemiology ; Cluster Analysis ; Disease Outbreaks ; Europe/epidemiology ; Hemagglutinins, Viral/genetics ; Humans ; Measles/epidemiology/transmission/virology ; Measles virus/classification/genetics/isolation & purification ; Molecular Epidemiology ; Molecular Sequence Data ; Molecular Typing ; Nucleoproteins/genetics ; Sequence Analysis, DNA ; Sequence Homology ; Viral Proteins/genetics
[en] With improved measles virus (MV) control, the genetic variability of the MV-nucleoprotein hypervariable region (NP-HVR) decreases. Thus, it becomes increasingly difficult to determine the origin of a virus using only this part of the genome. During outbreaks in Europe and Africa, we found MV strains with identical NP-HVR sequences. However, these strains showed considerable diversity within a larger sequencing window based on concatenated MV phosphoprotein and hemagglutinin genes (P/H pseudogenes). In Belarus, Germany, Russia, and the Democratic Republic of Congo, the P/H pseudogenes provided insights into chains of transmission, whereas identical NP-HVR provided none. In Russia, for instance, the P/H pseudogene identified temporal clusters rather than geographical clusters, demonstrating the circulation and importation of independent variants rather than large local outbreaks lasting for several years, as suggested by NP-HVR. Thus, by extending the sequencing window for molecular epidemiology, a more refined picture of MV circulation was obtained with more clearly defined links between outbreaks and transmission chains. Our results also suggested that in contrast to the P gene, the H gene acquired fixed substitutions that continued to be found in subsequent outbreaks, possibly with consequences for its antigenicity. Thus, a longer sequencing window has true benefits both for the epidemiological surveillance of measles and for the better monitoring of viral evolution.
http://hdl.handle.net/10993/3298

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