Reference : ΔNp63α expression induces loss of cell adhesion in triple-negative breast cancer cells
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/32912
ΔNp63α expression induces loss of cell adhesion in triple-negative breast cancer cells
English
Nekulova, M. [Regional Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, Zluty kopec 7, Brno, Czech Republic]
Holcakova, J. [Regional Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, Zluty kopec 7, Brno, Czech Republic]
Gu, X. [Department of Medical Biosciences, Umeå University, Umeå, Sweden]
Hrabal, V. [Regional Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, Zluty kopec 7, Brno, Czech Republic]
Galtsidis, Sotirios mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit]
Orzol, P. [Regional Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, Zluty kopec 7, Brno, Czech Republic]
Liu, Y. [NCRC, 026-329S, University of Michigan, Ann Arbor, MI, United States]
Logotheti, S. [Institute of Biology, Medicinal Chemistry and Biotechnology, NHRF, Athens, Greece]
Zoumpourlis, V. [Institute of Biology, Medicinal Chemistry and Biotechnology, NHRF, Athens, Greece]
Nylander, K. [Department of Medical Biosciences, Umeå University, Umeå, Sweden]
Coates, P. J. [Regional Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, Zluty kopec 7, Brno, Czech Republic]
Vojtesek, B. [Regional Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, Zluty kopec 7, Brno, Czech Republic]
2016
BMC Cancer
BioMed Central Ltd.
16
1
Yes (verified by ORBilu)
14712407
[en] Adhesion ; P63 isoforms ; Triple-negative breast cancer ; TP63 protein, human ; Article ; Animals ; Cell Adhesion ; Cell Cycle ; Cell Line, Tumor ; Cell Proliferation ; Cell Survival ; Cells, Cultured ; Disease Models, Animal ; Female ; Gene Expression ; Gene Expression Profiling ; Heterografts ; Humans ; Protein Isoforms ; Transcription Factors ; Triple Negative Breast Neoplasms ; Tumor Suppressor Proteins
[en] Background: p63, a member of the p53 protein family, plays key roles in epithelial development and carcinogenesis. In breast cancer, p63 expression has been found predominantly in basal-A (epithelial-type) triple-negative breast carcinomas (TNBC). To investigate the functional role of p63 in basal-A TNBC, we created MDA-MB-468 cell lines with inducible expression of the two major N-terminal p63 isoforms, TAp63α and ΔNp63α. Results: TAp63α did not have significant effect on gene expression profile and cell phenotype, whilst the main effect of ΔNp63α was reduction of cell adhesion. Gene expression profiling revealed genes involved in cell adhesion and migration whose expression relies on overexpression of ΔNp63α. Reduced cell adhesion also led to decreased cell proliferation in vitro and in vivo. Similar data were obtained in another basal-A cell line, BT-20, but not in BT-549 basal-B (mesenchymal-like) TNBC cells. Conclusions: In basal-A TNBC cells, ΔNp63α has much stronger effects on gene expression than TAp63α. Although p63 is mentioned mostly in connection with breast cell differentiation and stem cell regulation, we showed that a major effect of p63 is regulation of cell adhesion, a process important in metastasis and invasion of tumour cells. That this effect is not seen in mesenchymal-type TNBC cells suggests lineage-dependent functions, mirroring the expression of ΔNp63α in primary human breast cancers. © 2016 The Author(s).
http://hdl.handle.net/10993/32912
10.1186/s12885-016-2808-x

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