Reference : Integrated meta-omic analyses of the gastrointestinal tract microbiome in patients un...
Scientific journals : Article
Life sciences : Microbiology
Human health sciences : Hematology
http://hdl.handle.net/10993/32068
Integrated meta-omic analyses of the gastrointestinal tract microbiome in patients undergoing allogeneic hematopoietic stem cell transplantation.
English
Kaysen, Anne mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)]
Heintz-Buschart, Anna mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
Muller, Emilie mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
Narayanasamy, Shaman mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
Wampach, Linda mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
Laczny, Cedric C. mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)]
Graf, Norbert mailto [Saarland University Medical Center > Klinik für Pädiatrische Onkologie und Hämatologie]
Simon, Arne mailto [Saarland University Medical Center > Klinik für Pädiatrische Onkologie und Hämatologie]
Franke, Katharina mailto [> >]
Bittenbring, Jörg mailto [Saarland University Medical Center > Klinik für Innere Medizin I]
Wilmes, Paul mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
Schneider, Jochen mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
2017
Translational research : the journal of laboratory and clinical medicine
Yes
[en] In patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT), treatment-induced changes to the gastrointestinal tract (GIT) microbiome have been linked to adverse outcomes, most notably graft-versus-host disease (GvHD). However, it is presently unknown whether this relationship is causal or consequential. Here, we performed an integrated meta-omic analysis to probe deeper into the GIT microbiome changes during allo-HSCT and its accompanying treatments. We used 16S and 18S rRNA gene amplicon sequencing to resolve archaea, bacteria, and eukaryotes within the GIT microbiomes of 16 patients undergoing allo-HSCT for the treatment of hematologic malignancies. These results revealed a major shift in the GIT microbiome after allo-HSCT including a marked reduction in bacterial diversity, accompanied by only limited changes in eukaryotes and archaea. An integrated analysis of metagenomic and metatranscriptomic data was performed on samples collected from a patient before and after allo-HSCT for acute myeloid leukemia. This patient developed severe GvHD, leading to death 9 months after allo-HSCT. In addition to drastically decreased bacterial diversity, the post-treatment microbiome showed a higher overall number and higher expression levels of antibiotic resistance genes (ARGs). One specific Escherichia coli strain causing a paravertebral abscess was linked to GIT dysbiosis, suggesting loss of intestinal barrier integrity. The apparent selection for bacteria expressing ARGs suggests that prophylactic antibiotic administration may adversely affect the overall treatment outcome. We therefore assert that such analyses including information about the selection of pathogenic bacteria expressing ARGs may assist clinicians in "personalizing" regimens for individual patients to improve overall outcomes.
University of Luxembourg - UL ; Fonds National de la Recherche - FnR
http://hdl.handle.net/10993/32068
10.1016/j.trsl.2017.06.008
http://www.translationalres.com/article/S1931-5244(17)30069-5/fulltext

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