Reference : Colonization and Succession within the Human Gut Microbiome by Archaea, Bacteria, and...
Scientific journals : Article
Life sciences : Microbiology
Systems Biomedicine
http://hdl.handle.net/10993/31318
Colonization and Succession within the Human Gut Microbiome by Archaea, Bacteria, and Microeukaryotes during the First Year of Life
English
Wampach, Linda mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Heintz, Anna mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Hogan, Angela mailto [Integrated BioBank of Luxembourg]
Muller, Emilie mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Narayanasamy, Shaman mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Laczny, Cedric Christian mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Hugerth, Luisa mailto [KTH Royal Institute of Technology > Science for Life Laboratory > School of Biotechnology - Division of Gene Technology]
Bindl, Lutz mailto [Centre Hospitalier de Luxembourg]
Bottu, Jean mailto [Centre Hospitalier de Luxembourg]
Andersson, Anders mailto [KTH Royal Institute of Technology > Science for Life Laboratory > School of Biotechnology - Division of Gene Technology]
de Beaufort, Carine mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Wilmes, Paul mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
May-2017
Frontiers in Microbiology
Frontiers Research Foundation
Infant Gut Microbiota Colonization and Food Impact
Yes
International
1664-302X
Lausanne
Switzerland
[en] fungi ; succession ; delivery mode ; infant gut microbiome ; amplicon sequencing ; microbial colonization ; quantitative real-time PCR
[en] Perturbations to the colonization process of the human gastrointestinal tract have been suggested to result in adverse health effects later in life. Although much research has been performed on bacterial colonization and succession, much less is known about the other two domains of life, archaea, and eukaryotes. Here we describe colonization and succession by bacteria, archaea and microeukaryotes during the first year of life (samples collected around days 1, 3, 5, 28, 150, and 365) within the gastrointestinal tract of infants delivered either vaginally or by cesarean section and using a combination of quantitative real-time PCR as well as 16S and 18S rRNA gene amplicon sequencing. Sequences from organisms belonging to all three domains of life were detectable in all of the collected meconium samples. The microeukaryotic community composition fluctuated strongly over time and early diversification was delayed in infants receiving formula milk. Cesarean section-delivered (CSD) infants experienced a delay in colonization and succession, which was observed for all three domains of life. Shifts in prokaryotic succession in CSD infants compared to vaginally delivered (VD) infants were apparent as early as days 3 and 5, which were characterized by increased relative abundances of the genera Streptococcus and Staphylococcus, and a decrease in relative abundance for the genera Bifidobacterium and Bacteroides. Generally, a depletion in Bacteroidetes was detected as early as day 5 postpartum in CSD infants, causing a significantly increased Firmicutes/Bacteroidetes ratio between days 5 and 150 when compared to VD infants. Although the delivery mode appeared to have the strongest influence on differences between the infants, other factors such as a younger gestational age or maternal antibiotics intake likely contributed to the observed patterns as well. Our findings complement previous observations of a delay in colonization and succession of CSD infants, which affects not only bacteria but also archaea and microeukaryotes. This further highlights the need for resolving bacterial, archaeal, and microeukaryotic dynamics in future longitudinal studies of microbial colonization and succession within the neonatal gastrointestinal tract.
Luxembourg Centre for Systems Biomedicine (LCSB): Eco-Systems Biology (Wilmes Group) ; Integrated BioBank of Luxembourg - IBBL ; Science for Life Laboratory ; Centre Hospitalier de Luxembourg
Fonds National de la Recherche - FnR ; Integrated BioBank of Luxembourg ; Fondation André et Henriette Losch ; Swedish Research Council VR
Cosmic
http://hdl.handle.net/10993/31318
10.3389/fmicb.2017.00738
http://journal.frontiersin.org/article/10.3389/fmicb.2017.00738/full#h8
Copyright © 2017 Wampach, Heintz-Buschart, Hogan, Muller, Narayanasamy, Laczny, Hugerth, Bindl, Bottu, Andersson, de Beaufort and Wilmes. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
FnR ; FNR5824125 > Linda Belardi-Wampach > COSMIC-PHD1 > Colonisation, succession and evolution of the human gastrointestinal microbiome in infants at high risk of metabolic disease in adulthood > 01/10/2013 > 30/09/2017 > 2013

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
FINAL_fmicb-08-00738.pdfPublisher postprint2.76 MBView/Open

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.