Reference : Hind limb unloading, a model of spaceflight conditions, leads to decreased B lymphopo...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/31051
Hind limb unloading, a model of spaceflight conditions, leads to decreased B lymphopoiesis similar to aging
English
Lescale, Chloé [Institut Universitaire d'Hématologie, Université Paris 7 Denis Diderot, Paris, France > UMRS1126]
Schenten, Véronique mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit]
Djeghloul, Dounia [Institut Universitaire d'Hématologie, Université Paris 7 Denis Diderot, Paris, France > UMRS1126]
Bennabi, Meriem [Institut Universitaire d'Hématologie, Université Paris 7 Denis Diderot, Paris, France > UMRS1126]
Gaignier, Fanny [Lorraine University, Faculty of Medicine, 9 Avenue de la Forêt de Haye, F-54500 Vandœuvre-lès-Nancy, France > EA7300, Stress Immunity Pathogens Laboratory]
Vandamme, Katleen [Faculté de Médecine Lariboisière, Paris, France > UMR CNRS 7052, Biomécanique et Biomatériaux Ostéo-articulaires]
Strazielle, Catherine [Lorraine University, Faculty of Medicine, 9 Avenue de la Forêt de Haye, F-54500 Vandœuvre-lès-Nancy, France > EA7300, Stress Immunity Pathogens Laboratory]
Kuzniak, Isabelle [Institut Universitaire d'Hématologie, Université Paris 7 Denis Diderot, Paris, France > UMRS1126]
Petite, Hervé [Institut Universitaire d'Hématologie, Université Paris 7 Denis Diderot, Paris, France > UMRS1126]
Dosquet, Christine [Institut Universitaire d'Hématologie, Université Paris 7 Denis Diderot, Paris, France > UMRS1126]
Frippiat, Jean-Pol [Lorraine University, Faculty of Medicine, 9 Avenue de la Forêt de Haye, F-54500 Vandœuvre-lès-Nancy, France > EA7300, Stress Immunity Pathogens Laboratory]
Goodhardt, Michèle [Institut Universitaire d'Hématologie, Université Paris 7 Denis Diderot, Paris, France > UMRS1126]
2015
FASEB Journal
Federation of American Society for Experimental Biology
29
2
455-63
Yes (verified by ORBilu)
International
0892-6638
1530-6860
Bethesda
MD
[en] Adrenal Cortex Hormones ; Interleukin-7 ; PAX5 ; STAT5 ; Microtomography ; immunosenescence ; gravity
[en] Within the bone marrow, the endosteal niche plays a crucial role in B-cell differentiation. Because spaceflight is associated with osteoporosis, we investigated whether changes in bone microstructure induced by a ground-based model of spaceflight, hind limb unloading (HU), could affect B lymphopoiesis. To this end, we analyzed both bone parameters and the frequency of early hematopoietic precursors and cells of the B lineage after 3, 6, 13, and 21 d of HU. We found that limb disuse leads to a decrease in both bone microstructure and the frequency of B-cell progenitors in the bone marrow. Although multipotent hematopoietic progenitors were not affected by HU, a decrease in B lymphopoiesis was observed as of the common lymphoid progenitor (CLP) stage with a major block at the progenitor B (pro-B) to precursor B (pre-B) cell transition (5- to 10-fold decrease). The modifications in B lymphopoiesis were similar to those observed in aged mice and, as with aging, decreased B-cell generation in HU mice was associated with reduced expression of B-cell transcription factors, early B-cell factor (EBF) and Pax5, and an alteration in STAT5-mediated IL-7 signaling. These findings demonstrate that mechanical unloading of hind limbs results in a decrease in early B-cell differentiation resembling age-related modifications in B lymphopoiesis.
http://hdl.handle.net/10993/31051
10.1096/fj.14-259770
http://www.ncbi.nlm.nih.gov/pubmed/25376832

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