Reference : Effects of Endocrine Disruptor Compounds, Alone or in Combination, on Human Macrophag...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/31050
Effects of Endocrine Disruptor Compounds, Alone or in Combination, on Human Macrophage-Like THP-1 Cell Response
English
Couleau, Nicolas [Université de Lorraine, IUT Thionville-Yutz, Espace Cormontaigne, Yutz, France > CNRS UMR 7360 > Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC)]
Falla, Jairo [Université de Lorraine, IUT Thionville-Yutz, Espace Cormontaigne, Yutz, France > CNRS UMR 7360 > Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC)]
Beillerot, Adeline [IUT Thionville-Yutz, Impasse Alfred Kastler Espace Cormontaigne, Yutz, France]
Battaglia, Eric [Université de Lorraine, Campus Bridoux-8, Metz, France > CNRS UMR 7360 > Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC)]
d’Innocenzo, Marielle [IUT Thionville-Yutz, Impasse Alfred Kastler Espace Cormontaigne, Yutz, France]
Plançon, Sébastien mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Laval-Gilly, Philippe [Université de Lorraine, IUT Thionville-Yutz, Espace Cormontaigne, Yutz, France > CNRS UMR 7360 > Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC)]
Bennasroune, Amar mailto [Université de Lorraine, IUT Thionville-Yutz, Espace Cormontaigne, Yutz, France > CNRS UMR 7360 > Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC)]
2-Jul-2015
PLoS ONE
Public Library of Science
10
7
e0131428
Yes (verified by ORBilu)
International
1932-6203
San Franscisco
CA
[en] Cell differentiation ; Secretion ; Phagocytosis ; Cytokines ; Macrophages ; Estrogens ; Immune response
[en] The aim of the present study was to evaluate the immunological effects on human macrophages of four endocrine disruptor compounds (EDCs) using the differentiated human THP-1 cell line as a model. We studied first the effects of these EDCs, including Bisphenol A (BPA), di-ethylhexyl-phthalate (DEHP), dibutyl phthalate (DBP) and 4-tert-octylphenol (4-OP), either alone or in combination, on cytokine secretion, and phagocytosis. We then determined whether or not these effects were mediated by estrogen receptors via MAPK pathways. It was found that all four EDCs studied reduced strongly the phagocytosis of the differentiated THP-1 cells and that several of these EDCs disturbed also TNF-α, IL-1 β and IL-8 cytokine secretions. Furthermore, relative to control treatment, decreased ERK 1/2 phosphorylation was always associated with EDCs treatments-either alone or in certain combinations (at 0.1 μM for each condition). Lastly, as treatments by an estrogen receptor antagonist suppressed the negative effects on ERK 1/2 phosphorylation observed in cells treated either alone with BPA, DEHP, 4-OP or with the combined treatment of BPA and DEHP, we suggested that estrogen receptor-dependent pathway is involved in mediating the effects of EDCs on human immune system. Altogether, these results advocate that EDCs can disturb human immune response at very low concentrations.
http://hdl.handle.net/10993/31050

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