Reference : Spinal motor neurons are regenerated after mechanical lesion and genetic ablation in ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Genetics & genetic processes
Life sciences : Multidisciplinary, general & others
http://hdl.handle.net/10993/30442
Spinal motor neurons are regenerated after mechanical lesion and genetic ablation in larval zebrafish
English
Ohnmacht, Jochen* mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Yang, Yujie* []
Maurer, Giana []
Barreiro-Iglesias, Antón []
Tsarouchas, Themistoklis []
Wehner, Daniel []
Sieger, Dirk []
Becker, Catherina []
Becker, Thomas []
* These authors have contributed equally to this work.
1-May-2016
Development
Company of Biologists
143
9
1464-1474
Yes (verified by ORBilu)
International
0950-1991
1477-9129
Cambridge
United Kingdom
[en] In adult zebrafish, relatively quiescent progenitor cells show lesion-induced generation of motor neurons. Developmental motor neuron generation from the spinal motor neuron progenitor domain (pMN) sharply declines at 48 hours post-fertilisation (hpf). After that, mostly oligodendrocytes are generated from the same domain. We demonstrate here that within 48 h of a spinal lesion or specific genetic ablation of motor neurons at 72 hpf, the pMN domain reverts to motor neuron generation at the expense of oligodendrogenesis. By contrast, generation of dorsal Pax2-positive interneurons was not altered. Larval motor neuron regeneration can be boosted by dopaminergic drugs, similar to adult regeneration. We use larval lesions to show that pharmacological suppression of the cellular response of the innate immune system inhibits motor neuron regeneration. Hence, we have established a rapid larval regeneration paradigm. Either mechanical lesions or motor neuron ablation is sufficient to reveal a high degree of developmental flexibility of pMN progenitor cells. In addition, we show an important influence of the immune system on motor neuron regeneration from these progenitor cells.
Researchers ; Professionals
http://hdl.handle.net/10993/30442
10.1242/dev.129155

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
1464.full.pdfPublisher postprint4.55 MBView/Open

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.