Reference : Stable Isotope-Assisted Evaluation of Different Extraction Solvents for Untargeted Me...
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
http://hdl.handle.net/10993/28076
Stable Isotope-Assisted Evaluation of Different Extraction Solvents for Untargeted Metabolomics of Plants
English
Doppler, Maria mailto [University of Natural Resources and Life Siences, Vienna > Department of Agrobiotechnology > Center for analytical chemistry]
Kluger, Bernhard mailto [University of Natural Resources and Life Sciences, Vienna > Department of Agrobiotechnology > Center for Analytical Chemistry]
Bueschl, Christoph mailto [University of Natural Resources and Life Sciences, Vienna > Department of Agrobiotechnology > Center for Analytical Chemistry]
Schneider, Christina mailto [University of Natural Resources and Life Science, Vienna > Department of Agrobiotechnology > Center for Analytical Chemistry]
Krska, Rudolf mailto [University of Natural Resources and Life Sciences, Vienna > Department of Agrobiotechnology > Center for Analytical Chemistry]
Delcambre, Sylvie mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Hiller, Karsten mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Lemmens, Marc mailto [University of Natural Resources and Life Sciences, Vienna > Deparment of Agrobiotechnology > Center for Analytical Chemistry]
Schuhmacher, Rainer mailto [University of Nattural Resources and Life Sciences, Vienna > Department of Agrobiotechnology > Center for Analytical Chemistry]
28-Jun-2016
International Journal of Molecular Sciences
Yes
[en] 13 C-labelling ; plant metabolomics ; sample preparation ; Triticum aestivum ; wheat ; untargeted metabolomics
[en] The evaluation of extraction protocols for untargeted metabolomics approaches is still difficult. We have applied a novel stable isotope-assisted workflow for untargeted LC-HRMS-based plant metabolomics , which allows for the first time every detected feature to be considered for method evaluation. The efficiency and complementarity of commonly used extraction solvents, namely 1 + 3 (v/v) mixtures of water and selected organic solvents (methanol, acetonitrile or methanol/acetonitrile 1 + 1 (v/v)), with and without the addition of 0.1% (v/v) formic acid were compared. Four different wheat organs were sampled, extracted and analysed by LC-HRMS. Data evaluation was performed with the in-house-developed MetExtract II software and R. With all tested solvents a total of 871 metabolites were extracted in ear, 785 in stem, 733 in leaf and 517 in root samples, respectively. Between 48% (stem) and 57% (ear) of the metabolites detected in a particular organ were found with all extraction mixtures, and 127 of 996 metabolites were consistently shared between all extraction agent/organ combinations. In aqueous methanol, acidification with formic acid led to pronounced pH dependency regarding the precision of metabolite abundance and the number of detectable metabolites, whereas extracts of acetonitrile-containing mixtures were less affected. Moreover, methanol and acetonitrile have been found to be complementary with respect to extraction efficiency. Interestingly, the beneficial properties of both solvents can be combined by the use of a water-methanol-acetonitrile mixture for global metabolite extraction instead of aqueous methanol or aqueous acetonitrile alone.
Austrian Science Fund (project SFB-Fusarium-3706-B11;3702) ; Lower Austria (Project NovAlgo) ; Fonds National de la Recherche (FNR), Luxembourg (ATTRACT A10/03, THActivity CORE and AF-PhD-5782260)
http://hdl.handle.net/10993/28076
10.3390

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