Reference : Hyperosmotic stress induces immediate-early gene expression in ventricular adult card...
Scientific journals : Article
Human health sciences : Cardiovascular & respiratory systems
http://hdl.handle.net/10993/27502
Hyperosmotic stress induces immediate-early gene expression in ventricular adult cardiomyocytes.
English
Wollnik, B. [> >]
Kubisch, C. [> >]
Maass, A. [> >]
Vetter, H. [> >]
Neyses, Ludwig mailto [University of Luxembourg > Research Office]
1993
Biochemical and biophysical research communications
194
2
642-6
Yes
International
0006-291X
UNITED STATES
[en] Animals ; Blotting, Northern ; Cells, Cultured ; DNA-Binding Proteins/biosynthesis/genetics ; Early Growth Response Protein 1 ; Gene Expression Regulation/drug effects ; Genes, fos ; Heart Ventricles ; Immediate-Early Proteins ; Male ; Myocardium/cytology/metabolism ; Proto-Oncogenes ; RNA, Messenger/biosynthesis/isolation & purification/metabolism ; Rats ; Rats, Inbred WKY ; Saline Solution, Hypertonic/pharmacology ; Transcription Factors/biosynthesis/genetics ; Zinc Fingers/genetics
[en] Mammalian cells possessing osmosensors have long been described in brain and kidney. The genetic basis of the response to hyperosmotic stress has been well characterized in prokaryotes. In contrast, the genetic response of eukaryotic cells is poorly understood. Therefore we investigated the effect of hypertonic NaCl and sucrose solutions on the transcriptional activation of the immediate-early genes (IEGs) egr-1 and c-fos in isolated ventricular adult rat cardiomyocytes. We observed that even small increases in osmolarity to 315 +/- 5 mosmol/l and 370 +/- 8 mosmol/l by hypertonic NaCl solution resulted in dose-dependent induction of egr-1 (4-and 5-fold) and c-fos (3-and 4-fold), respectively. Hypertonic sucrose solution had the same effect on egr-1 and c-fos mRNA levels while increased sucrose concentration under isotonic conditions had no effect. Cardiomyocytes exposed to hypertonic media did not significantly shrink as shown by a cell length measurement. We conclude that isolated adult cardiomyocytes possess an osmoreceptor mechanism which is able to sense even slight changes in osmolarity and to translate these into a transcriptional response of the myocardial IEG program.
http://hdl.handle.net/10993/27502
10.1006/bbrc.1993.1869
http://www.sciencedirect.com/science/article/pii/S0006291X83718693

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