Reference : A microtubule-independent role of p150glued in secretory cargo concentration at endop...
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/10993/27424
A microtubule-independent role of p150glued in secretory cargo concentration at endoplasmic reticulum exit sites
English
Verissimo, Fatima [Cell Biology and Biophysics Unit, EMBL, Meyerhofstraße 1, Heidelberg, Germany]
Halavatyi, Aliaksandr mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit]
Pepperkok, Rainer [Cell Biology and Biophysics Unit, EMBL, Meyerhofstraße 1, Heidelberg, Germany]
Weiss, Matthias [Experimental Physics I, University of Bayreuth, Universitaetsstr. 30, Bayreuth, Germany]
2015
Journal of Cell Science
Company of Biologists Ltd
128
22
4160-4170
Yes (verified by ORBilu)
International
00219533
[en] Endoplasmic reticulum ; ER exit sites ; FRAP ; Microtubules ; Modeling ; Protein sorting ; Secretory pathway ; Article ; Golgi complex ; HeLa cell line
[en] Newly synthesized proteins are sorted into COPII-coated transport carriers at the endoplasmic reticulum (ER). Assembly of the COPII coat complex, which occurs at ER exit sites (ERES), is initiated by membrane association and GTP loading of SAR1, followed by the recruitment of the SEC23-SEC24 and SEC13-SEC31 subcomplexes. Both of these two subcomplexes stimulate GTP hydrolysis and coat disassembly. This inherent disassembly capacity of COPII complexes needs to be regulated to allow sufficient time for cargo sorting and transport carrier formation. By performing fluorescence recovery after photobleaching (FRAP) and mathematical modeling, we show that p150glued (also known as DCTN1), a component of the dynactin complex, stabilizes the COPII pre-budding complex on ER membranes in a microtubule-independent manner. Concentration of the secretory marker ts-O45-G at ERES is reduced in the presence of a C-terminal p150glued fragment that prevents binding of endogenous p150glued to SEC23. A similar cargo reduction is observed upon p150glued knockdown. Taken together, our data suggest that cargo concentration at ERES is regulated by p150glued to coordinate protein sorting and transport carrier formation with the subsequent long-range transport towards the Golgi complex along microtubules. © 2015. Published by The Company of Biologists Ltd.
RGY0076/2010, HFSP, Human Frontier Science Program
http://hdl.handle.net/10993/27424
10.1242/jcs.172395

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