Reference : Broad RNA interference-mediated antiviral immunity and virus-specific inducible respo...
Scientific journals : Article
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/10993/27299
Broad RNA interference-mediated antiviral immunity and virus-specific inducible responses in Drosophila
English
Kemp, Cordula [CNRS-UPR9022, Institut de Biologie Moléculaire et Cellulaire, 67084 Strasbourg Cedex, France]
Mueller, Stefanie [CNRS-UPR9022, Institut de Biologie Moléculaire et Cellulaire, 67084 Strasbourg Cedex, France, Bernhard-Nocht-Institut for Tropical Medicine, Molecular Entomology, Hamburg, Germany]
Goto, Akira [CNRS-UPR9022, Institut de Biologie Moléculaire et Cellulaire, 67084 Strasbourg Cedex, France]
Barbier, Vincent [CNRS-UPR9022, Institut de Biologie Moléculaire et Cellulaire, 67084 Strasbourg Cedex, France]
Paro, Simona [CNRS-UPR9022, Institut de Biologie Moléculaire et Cellulaire, 67084 Strasbourg Cedex, France]
Bonnay, François [CNRS-UPR9022, Institut de Biologie Moléculaire et Cellulaire, 67084 Strasbourg Cedex, France]
Dostert, Catherine mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit]
Troxler, Laurent [CNRS-UPR9022, Institut de Biologie Moléculaire et Cellulaire, 67084 Strasbourg Cedex, France]
Hetru, Charles [CNRS-UPR9022, Institut de Biologie Moléculaire et Cellulaire, 67084 Strasbourg Cedex, France]
Meignin, Carine [CNRS-UPR9022, Institut de Biologie Moléculaire et Cellulaire, 67084 Strasbourg Cedex, France]
Pfeffer, Sébastien [CNRS-UPR9002, Institut de Biologie Moléculaire et Cellulaire, 67084 Strasbourg Cedex, France]
Hoffmann, Jules A. [CNRS-UPR9022, Institut de Biologie Moléculaire et Cellulaire, 67084 Strasbourg Cedex, France]
Imler, Jean-Luc [CNRS-UPR9022, Institut de Biologie Moléculaire et Cellulaire, 67084 Strasbourg Cedex, France, Faculté des Sciences de la Vie, Université de Strasbourg, 67083 Strasbourg Cedex, France]
2013
Journal of Immunology
190
2
650-658
Yes (verified by ORBilu)
International
00221767
[en] Janus kinase ; Birnaviridae ; Dicistroviridae ; DNA virus ; Drosophila ; Flock House virus ; Iridovirus ; Rhabdovirus ; RNA analysis ; RNA interference ; RNA sequence ; RNA virus ; Sindbis virus ; Alphavirus ; Alphavirus Infections ; Animals ; Animals, Genetically Modified ; DNA Virus Infections ; Drosophila melanogaster ; Drosophila Proteins ; Gene Expression Regulation ; Janus Kinases ; Male ; Nodaviridae ; Ribonuclease III ; RNA Helicases ; RNA Interference ; RNA Virus Infections ; Transcription Factors
[en] The fruit fly Drosophila melanogaster is a good model to unravel the molecular mechanisms of innate immunity and has led to some important discoveries about the sensing and signaling of microbial infections. The response of Drosophila to virus infections remains poorly characterized and appears to involve two facets. On the one hand, RNA interference involves the recognition and processing of dsRNA into small interfering RNAs by the host RNase Dicer-2 (Dcr-2), whereas, on the other hand, an inducible response controlled by the evolutionarily conserved JAK-STAT pathway contributes to the antiviral host defense. To clarify the contribution of the small interfering RNA and JAK-STAT pathways to the control of viral infections, we have compared the resistance of flies wild-type and mutant for Dcr-2 or the JAK kinase Hopscotch to infections by seven RNA or DNA viruses belonging to different families. Our results reveal a unique susceptibility of hop mutant flies to infection by Drosophila C virus and cricket paralysis virus, two members of the Dicistroviridae family, which contrasts with the susceptibility of Dcr-2 mutant flies to many viruses, including the DNA virus invertebrate iridescent virus 6. Genome-wide microarray analysis confirmed that different sets of genes were induced following infection by Drosophila C virus or by two unrelated RNA viruses, Flock House virus and Sindbis virus. Overall, our data reveal that RNA interference is an efficient antiviral mechanism, operating against a large range of viruses, including a DNA virus. By contrast, the antiviral contribution of the JAK-STAT pathway appears to be virus specific. Copyright © 2013 by The American Association of Immunologists, Inc.
http://hdl.handle.net/10993/27299
10.4049/jimmunol.1102486

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