Reference : Optimization and Pharmacological Validation of a Leukocyte Migration Assay in Zebrafi...
Scientific journals : Article
Human health sciences : Multidisciplinary, general & others
http://hdl.handle.net/10993/27291
Optimization and Pharmacological Validation of a Leukocyte Migration Assay in Zebrafish Larvae for the Rapid In Vivo Bioactivity Analysis of Anti-Inflammatory Secondary Metabolites
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Cordero-Maldonado, M. L. [Laboratory for Molecular Biodiscovery, Department of Pharmaceutical and Pharmacological Sciences, University of Leuven, Leuven, Belgium, School of Biochemistry and Pharmacy, University of Cuenca, Cuenca, Ecuador, Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg]
Siverio-Mota, D. [Laboratory for Molecular Biodiscovery, Department of Pharmaceutical and Pharmacological Sciences, University of Leuven, Leuven, Belgium, Department of Pharmacy, Central University Marta Abreu of Las Villas, Santa Clara, Cuba]
Vicet-Muro, L. [Laboratory for Molecular Biodiscovery, Department of Pharmaceutical and Pharmacological Sciences, University of Leuven, Leuven, Belgium, Department of Pharmacy, Central University Marta Abreu of Las Villas, Santa Clara, Cuba]
Wilches-Arizábala, I. M. [School of Biochemistry and Pharmacy, University of Cuenca, Cuenca, Ecuador]
Esguerra, C. V. [Laboratory for Molecular Biodiscovery, Department of Pharmaceutical and Pharmacological Sciences, University of Leuven, Leuven, Belgium]
de Witte, P. A. M. [Laboratory for Molecular Biodiscovery, Department of Pharmaceutical and Pharmacological Sciences, University of Leuven, Leuven, Belgium]
Crawford, Alexander Dettmar mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
2013
PLoS ONE
8
10
Yes (verified by ORBilu)
19326203
[en] Arnica montana extract ; Capraria biflora extract ; Chamaemelum nobile extract ; Harpagophytum procumbens extract ; Matricaria recutita extract ; Schefflera chimbuensis extract ; Syzygium montanum extract ; Araliaceae ; Arnica montana ; Calendula officinalis ; Capraria biflora ; Chamaemelum ; Harpagophytum ; Matricaria ; Schefflera chimbuensis ; Scrophulariaceae ; Syzygium ; Syzygium montanum ; Animals ; Anti-Inflammatory Agents ; Cell Migration Assays, Leukocyte ; Inflammation ; Larva ; Lipopolysaccharides ; Zebrafish
[en] Over the past decade, zebrafish (Danio rerio) have emerged as an attractive model for in vivo drug discovery. In this study, we explore the suitability of zebrafish larvae to rapidly evaluate the anti-inflammatory activity of natural products (NPs) and medicinal plants used in traditional medicine for the treatment of inflammatory disorders. First, we optimized a zebrafish assay for leukocyte migration. Inflammation was induced in four days post-fertilization (dpf) zebrafish larvae by tail transection and co-incubation with bacterial lipopolysaccharides (LPS), resulting in a robust recruitment of leukocytes to the zone of injury. Migrating zebrafish leukocytes were detected in situ by myeloperoxidase (MPO) staining, and anti-inflammatory activity was semi-quantitatively scored using a standardized scale of relative leukocyte migration (RLM). Pharmacological validation of this optimized assay was performed with a panel of anti-inflammatory drugs, demonstrating a concentration-responsive inhibition of leukocyte migration for both steroidal and non-steroidal anti-inflammatory drugs (SAIDs and NSAIDs). Subsequently, we evaluated the bioactivity of structurally diverse NPs with well-documented anti-inflammatory properties. Finally, we further used this zebrafish-based assay to quantify the anti-inflammatory activity in the aqueous and methanolic extracts of several medicinal plants. Our results indicate the suitability of this LPS-enhanced leukocyte migration assay in zebrafish larvae as a front-line screening platform in NP discovery, including for the bioassay-guided isolation of anti-inflammatory secondary metabolites from complex NP extracts. © 2013 Cordero-Maldonado et al.
http://hdl.handle.net/10993/27291
10.1371/journal.pone.0075404

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