Reference : A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Scientific journals : Article
Life sciences : Biotechnology
Life sciences : Microbiology
Life sciences : Multidisciplinary, general & others
Human health sciences : Laboratory medicine & medical technology
Systems Biomedicine
http://hdl.handle.net/10993/27053
A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
English
Shah, Pranjul mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
Fritz, Joëlle mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Glaab, Enrico mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
Desai, Mahesh S. [> >]
Greenhalgh, Kacy mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
Frachet Bour, Audrey mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Niegowska, Magdalena [> >]
Estes, Matthew [> >]
Jäger, Christian mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
Seguin-Devaux, Carole [> >]
Zenhausern, Frederic [> >]
Wilmes, Paul mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
2016
Nature communications
Nature Pub.lishing Group
7
11535
Yes (verified by ORBilu)
International
2041-1723
2041-1723
London
England
[en] microfluidics ; microbiome ; co-culture ; gastrointestinal ; model ; microarray analysis ; confocal microscopy ; PCR
[en] Changes in the human gastrointestinal microbiome are associated with several diseases. To infer causality, experiments in representative models are essential, but widely used animal models exhibit limitations. Here we present a modular, microfluidics-based model (HuMiX, human-microbial crosstalk), which allows co-culture of human and microbial cells under conditions representative of the gastrointestinal human-microbe interface. We demonstrate the ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions. In addition, we show that the co-culture of human epithelial cells with the obligate anaerobe Bacteroides caccae and LGG results in a transcriptional response, which is distinct from that of a co-culture solely comprising LGG. HuMiX facilitates investigations of host-microbe molecular interactions and provides insights into a range of fundamental research questions linking the gastrointestinal microbiome to human health and disease.
Luxembourg Centre for Systems Biomedicine (LCSB): Eco-Systems Biology (Wilmes Group)
Fonds National de la Recherche Luxembourg - FNR
Researchers ; Professionals ; Students
http://hdl.handle.net/10993/27053
also: http://hdl.handle.net/10993/27382
10.1038/ncomms11535
http://www.nature.com/ncomms/2016/160511/ncomms11535/full/ncomms11535.html
The original publication is available at http://www.nature.com/ncomms/2016/160511/ncomms11535/full/ncomms11535.html
FNR ; FNR11014639 > Paul Wilmes > HuMiX2.0 > A microfluidics-based drug discovery platform emulating the human microbiome on chip > 01/09/2015 > 31/12/2016 > 2015

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