Reference : The complement regulator CD46 is bactericidal to Helicobacter pylori and blocks ureas...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/26509
The complement regulator CD46 is bactericidal to Helicobacter pylori and blocks urease activity
English
Basmarke-Wehelie, Rahma [> >]
Sjolinder, Hong [> >]
Jurkowski, Wiktor [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Elofsson, Arne [> >]
Arnqvist, Anna [> >]
Engstrand, Lars [> >]
Hagner, Matthias [> >]
Wallin, Elin [> >]
Guan, Na [> >]
Kuranasekera, Hasanthi [> >]
Aro, Helena [> >]
Jonsson, Ann-Beth [> >]
2011
Gastroenterology
W.B. Saunders
141
3
918-28
Yes (verified by ORBilu)
0016-5085
1528-0012
Philadelphia
PA
[en] BACKGROUND & AIMS: CD46 is a C3b/C4b binding complement regulator and a receptor for several human pathogens. We examined the interaction between CD46 and Helicobacter pylori (a bacterium that colonizes the human gastric mucosa and causes gastritis), peptic ulcers, and cancer. METHODS: Using gastric epithelial cells, we analyzed a set of H pylori strains and mutants for their ability to interact with CD46 and/or influence CD46 expression. Bacterial interaction with full-length CD46 and small CD46 peptides was evaluated by flow cytometry, fluorescence microscopy, enzyme-linked immunosorbent assay, and bacterial survival analyses. RESULTS: H pylori infection caused shedding of CD46 into the extracellular environment. A soluble form of CD46 bound to H pylori and inhibited growth, in a dose- and time-dependent manner, by interacting with urease and alkyl hydroperoxide reductase, which are essential bacterial pathogenicity-associated factors. Binding of CD46 or CD46-derived synthetic peptides blocked the urease activity and ability of bacteria to survive in acidic environments. Oral administration of one CD46 peptide eradicated H pylori from infected mice. CONCLUSIONS: CD46 is an antimicrobial agent that can eradicate H pylori. CD46 peptides might be developed to treat H pylori infection.
http://hdl.handle.net/10993/26509
10.1053/j.gastro.2011.05.009

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