Reference : Structural and mechanistic insights into Helicobacter pylori NikR activation.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/26456
Structural and mechanistic insights into Helicobacter pylori NikR activation.
English
Bahlawane, Christelle mailto [Univ Grenoble 1 > Lab Chim & Biol Metaux]
Dian, C. [European Synchrotron Radiat Facil]
Muller, Christian mailto [Inst Pasteur > Unite Pathogenese Helicobacter]
Round, A. [European Mol Biol Lab]
Fauquant, C. [Univ Grenoble 1 > Lab Chim & Biol Metaux]
Schauer, K. [Inst Pasteur > Unite Pathogenese Helicobacter]
de Reuse, H. [Inst Pasteur > Unite Pathogenese Helicobacter]
Terradot, L. [European Synchrotron Radiat Facil]
Michaud-Soret, I. [Univ Grenoble 1 > Lab Chim & Biol Metaux]
2010
Nucleic acids research
38
9
3106-18
Yes (verified by ORBilu)
International
0305-1048
1362-4962
England
[en] Bacterial Proteins/chemistry/genetics/metabolism ; DNA-Binding Proteins/chemistry/metabolism ; Models, Molecular ; Mutation ; Nickel/metabolism ; Protein Binding ; Repressor Proteins/chemistry/genetics/metabolism ; Scattering, Small Angle ; X-Ray Diffraction
[en] NikR is a transcriptional metalloregulator central in the mandatory response to acidity of Helicobacter pylori that controls the expression of numerous genes by binding to specific promoter regions. NikR/DNA interactions were proposed to rely on protein activation by Ni(II) binding to high-affinity (HA) and possibly secondary external (X) sites. We describe a biochemical characterization of HpNikR mutants that shows that the HA sites are essential but not sufficient for DNA binding, while the secondary external (X) sites and residues from the HpNikR dimer-dimer interface are important for DNA binding. We show that a second metal is necessary for HpNikR/DNA binding, but only to some promoters. Small-angle X-ray scattering shows that HpNikR adopts a defined conformation in solution, resembling the cis-conformation and suggests that nickel does not trigger large conformational changes in HpNikR. The crystal structures of selected mutants identify the effects of each mutation on HpNikR structure. This study unravels key structural features from which we derive a model for HpNikR activation where: (i) HA sites and an hydrogen bond network are required for DNA binding and (ii) metallation of a unique secondary external site (X) modulates HpNikR DNA binding to low-affinity promoters by disruption of a salt bridge.
http://hdl.handle.net/10993/26456

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