Reference : Antioxidant cytoprotection by peroxisomal peroxiredoxin-5.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/26377
Antioxidant cytoprotection by peroxisomal peroxiredoxin-5.
English
Walbrecq, Geoffroy mailto [Université Catholique de Louvain - UCL > Group ofAnimalMolecularandCellularBiology, InstitutdesSciencesdelaVie]
Wang, Bo [> >]
Becker, Sarah [> >]
Hannotiau, Amandine [> >]
Fransen, Marc [> >]
Knoops, Bernard [> >]
2015
Free radical biology & medicine
84
215-26
Yes (verified by ORBilu)
0891-5849
1873-4596
United States
[en] Animals ; Antioxidants/metabolism ; Cytoprotection ; Gene Expression ; Humans ; Lipid Peroxidation ; Mice ; Mitochondria ; Oligodendroglia/physiology ; Oxidative Stress ; Peroxiredoxins/physiology ; Peroxisomes/enzymology ; Free radicals ; KillerRed ; Lipid peroxidation ; Mitochondria ; Oxidative stress ; Peroxiredoxin-5 ; Peroxisomes ; roGFP2
[en] Peroxiredoxin-5 (PRDX5) is a thioredoxin peroxidase that reduces hydrogen peroxide, alkyl hydroperoxides, and peroxynitrite. This enzyme is present in the cytosol, mitochondria, peroxisomes, and nucleus in human cells. Antioxidant cytoprotective functions have been previously documented for cytosolic, mitochondrial, and nuclear mammalian PRDX5. However, the exact function of PRDX5 in peroxisomes is still not clear. The aim of this work was to determine the function of peroxisomal PRDX5 in mammalian cells and, more specifically, in glial cells. To study the role of PRDX5 in peroxisomes, the endogenous expression of PRDX5 in murine oligodendrocyte 158N cells was silenced by RNA interference. In addition, human PRDX5 was also overexpressed in peroxisomes using a vector coding for human PRDX5, whose unconventional peroxisomal targeting sequence 1 (PTS1; SQL) was replaced by the prototypical PTS1 SKL. Stable 158N clones were obtained. The antioxidant cytoprotective function of peroxisomal PRDX5 against peroxisomal and mitochondrial KillerRed-mediated reactive oxygen species production as well as H2O2 was examined using MTT viability assays, roGFP2, and C11-BOBIPY probes. Altogether our results show that peroxisomal PRDX5 protects 158N oligodendrocytes against peroxisomal and mitochondrial KillerRed- and H2O2-induced oxidative stress.
http://hdl.handle.net/10993/26377
Copyright (c) 2015 Elsevier Inc. All rights reserved.

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