Reference : SCNT-derived ESCs with mismatched mitochondria trigger an immune response in allogene...
Scientific journals : Article
Human health sciences : Laboratory medicine & medical technology
http://hdl.handle.net/10993/24456
SCNT-derived ESCs with mismatched mitochondria trigger an immune response in allogeneic hosts.
English
Deuse, Tobias [> >]
Wang, Dong [> >]
Stubbendorff, Mandy [> >]
Itagaki, Ryo [> >]
Grabosch, Antje [> >]
Greaves, Laura C. [> >]
Alawi, Malik [> >]
Grünewald, Anne [> >]
Hu, Xiaomeng [> >]
Hua, Xiaoqin [> >]
Velden, Joachim [> >]
Reichenspurner, Hermann [> >]
Robbins, Robert C. [> >]
Jaenisch, Rudolf [> >]
Weissman, Irving L. [> >]
Schrepfer, Sonja [> >]
2015
Cell Stem Cell
16
1
33-8
Yes (verified by ORBilu)
International
1875-9777
United States
[en] Animals ; Antigens/immunology ; Embryonic Stem Cells/cytology/metabolism ; Humans ; Immunity ; Mice, Inbred BALB C ; Mitochondria/metabolism ; Nuclear Transfer Techniques ; Transplantation, Homologous
[en] The generation of pluripotent stem cells by somatic cell nuclear transfer (SCNT) has recently been achieved in human cells and sparked new interest in this technology. The authors reporting this methodical breakthrough speculated that SCNT would allow the creation of patient-matched embryonic stem cells, even in patients with hereditary mitochondrial diseases. However, herein we show that mismatched mitochondria in nuclear-transfer-derived embryonic stem cells (NT-ESCs) possess alloantigenicity and are subject to immune rejection. In a murine transplantation setup, we demonstrate that allogeneic mitochondria in NT-ESCs, which are nucleus-identical to the recipient, may trigger an adaptive alloimmune response that impairs the survival of NT-ESC grafts. The immune response is adaptive, directed against mitochondrial content, and amenable for tolerance induction. Mitochondrial alloantigenicity should therefore be considered when developing therapeutic SCNT-based strategies.
Researchers ; Students
http://hdl.handle.net/10993/24456
Copyright (c) 2015 Elsevier Inc. All rights reserved.

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