Reference : PINK1 loss-of-function mutations affect mitochondrial complex I activity via NdufA10 ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/24448
PINK1 loss-of-function mutations affect mitochondrial complex I activity via NdufA10 ubiquinone uncoupling.
English
Morais, Vanessa A. [> >]
Haddad, Dominik [> >]
Craessaerts, Katleen [> >]
De Bock, Pieter-Jan [> >]
Swerts, Jef [> >]
Vilain, Sven [> >]
Aerts, Liesbeth [> >]
Overbergh, Lut [> >]
Grünewald, Anne [> >]
Seibler, Philip [> >]
Klein, Christine [> >]
Gevaert, Kris [> >]
Verstreken, Patrik [> >]
De Strooper, Bart [> >]
2014
Science (New York, N.Y.)
344
6180
203-7
Yes (verified by ORBilu)
0036-8075
1095-9203
United States
[en] Amino Acid Sequence ; Animals ; Brain/enzymology ; Drosophila Proteins/metabolism ; Electron Transport Complex I/metabolism ; Humans ; Liver/enzymology ; Membrane Potential, Mitochondrial/genetics ; Mice ; Mice, Knockout ; Molecular Sequence Data ; Mutation ; NADH Dehydrogenase/metabolism ; Parkinson Disease/enzymology/genetics ; Phosphorylation/genetics ; Protein Kinases/genetics ; Proteome ; Serine/chemistry/metabolism
[en] Under resting conditions, Pink1 knockout cells and cells derived from patients with PINK1 mutations display a loss of mitochondrial complex I reductive activity, causing a decrease in the mitochondrial membrane potential. Analyzing the phosphoproteome of complex I in liver and brain from Pink1(-/-) mice, we found specific loss of phosphorylation of serine-250 in complex I subunit NdufA10. Phosphorylation of serine-250 was needed for ubiquinone reduction by complex I. Phosphomimetic NdufA10 reversed Pink1 deficits in mouse knockout cells and rescued mitochondrial depolarization and synaptic transmission defects in pink(B9)-null mutant Drosophila. Complex I deficits and adenosine triphosphate synthesis were also rescued in cells derived from PINK1 patients. Thus, this evolutionary conserved pathway may contribute to the pathogenic cascade that eventually leads to Parkinson's disease in patients with PINK1 mutations.
Researchers ; Students
http://hdl.handle.net/10993/24448

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