Reference : Regulation of the human cyclin C gene via multiple vitamin D3-responsive regions in i...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/230
Regulation of the human cyclin C gene via multiple vitamin D3-responsive regions in its promoter
English
Sinkkonen, Lasse mailto [University of Kuopio > Faculty of Natural Sciences > Department of Biochemistry]
Malinen, Marjo [University of Kuopio > Faculty of Natural Sciences > Department of Biochemistry]
Saavalainen, Katri [University of Kuopio > Faculty of Natural Sciences > Department of Biochemistry]
Väisänen, Sami [University of Kuopio > Faculty of Natural Sciences > Department of Biochemistry]
Carlberg, Carsten [University of Kuopio > Faculty of Natural Sciences > Department of Biochemistry]
29-Apr-2005
Nucleic Acids Research
Oxford University Press
33
8
2440-51
Yes (verified by ORBilu)
International
0305-1048
1362-4962
Oxford
United Kingdom
[en] genetics ; biosynthesis ; metabolism ; analysis ; genetics ; pharmacology ; biosynthesis ; Steroid Hydroxylases ; Gene Expression Regulation ; Retinoid X Receptors ; Response Elements ; Receptors, Calcitriol ; Promoter Regions (Genetics) ; Humans ; Histones ; Acetylation ; Cytochrome P-450 Enzyme System ; Calcitriol ; Cyclins ; Cell Line, Tumor
[en] The candidate human tumor suppressor gene cyclin C is a primary target of the anti-proliferative hormone 1alpha,25-dihydroxyvitamin D3 [1alpha,25(OH)2D3], but binding sites for the 1alpha,25(OH)2D3 receptor (VDR), so-called 1alpha,25(OH)2D3 response elements (VDREs), have not yet been identified in the promoter of this gene. We screened various cancer cell lines by quantitative PCR and found that the 1alpha,25(OH)2D3 inducibility of cyclin C mRNA expression, in relationship with the 24-hydroxylase (CYP24) gene, was best in MCF-7 human breast cancer cells. To characterize the molecular mechanisms, we analyzed 8.4 kb of the cyclin C promoter by using chromatin immunoprecipitation assays (ChIP) with antibodies against acetylated histone 4, VDR and its partner receptor, retinoid X receptor (RXR). The histone 4 acetylation status of all 23 investigated regions of the cyclin C promoter did not change significantly in response to 1alpha,25(OH)2D3, but four independent promoter regions showed a consistent, 1alpha,25(OH)2D3-dependent association with VDR and RXR over a time period of 240 min. Combined in silico/in vitro screening identified in each of these promoter regions a VDRE and reporter gene assays confirmed their functionality. Moreover, re-ChIP assays monitored simultaneous association of VDR with RXR, coactivator, mediator and RNA polymerase II proteins on these regions. Since cyclin C protein is associated with those mediator complexes that display transcriptional repressive properties, this study contributes to the understanding of the downregulation of a number of secondary 1alpha,25(OH)2D3-responding genes.
http://hdl.handle.net/10993/230
also: http://hdl.handle.net/10993/278
10.1093/nar/gki502

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