Reference : Transcriptomic analyses of primary astrocytes under TNFα treatment
Scientific journals : Article
Life sciences : Biotechnology
Life sciences : Multidisciplinary, general & others
Physical, chemical, mathematical & earth Sciences : Multidisciplinary, general & others
Human health sciences : Neurology
http://hdl.handle.net/10993/22485
Transcriptomic analyses of primary astrocytes under TNFα treatment
English
Birck, Cindy [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Koncina, Eric [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Heurtaux, Tony [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Glaab, Enrico mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Michelucci, Alessandro [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Heuschling, Paul [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Grandbarbe, Luc [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
2016
Genomics Data
Elsevier
7
7-11
Yes
International
2213-5960
Amsterdam
The Netherlands
[en] primary astrocytes ; bioinformatics ; TNF-alpha ; inflammation ; brain ; NFkappaB ; microarray ; gene expression
[en] Astrocytes, the most abundant glial cell population in the central nervous system, have important functional roles in the brain as blood brain barrier maintenance, synaptic transmission or intercellular communications. Numerous studies suggested that astrocytes exhibit a functional and morphological high degree of plasticity. For example, following any brain injury, astrocytes become reactive and hypertrophic. This phenomenon, also called reactive gliosis, is characterized by a set of progressive gene expression and cellular changes. Interestingly, in this context, astrocytes can re-acquire neurogenic properties. It has been shown that astrocytes can undergo dedifferentiation upon injury and inflammation, and may re-acquire the potentiality of neural progenitors.

To assess the effect of inflammation on astrocytes, primary mouse astrocytes were treated with tumor necrosis factor α (TNFα), one of the main pro-inflammatory cytokines. The strength of this study is that pure primary astrocytes were used. As microglia are highly reactive immune cells, we used a magnetic cell sorting separation (MACS) method to further obtain highly pure astrocyte cultures devoid of microglia.

Here, we provide details of the microarray data, which have been deposited in the Gene Expression Omnibus (GEO) under the series accession number GSE73022. The analysis and interpretation of these data are included in Gabel et al. (2015). Analysis of gene expression indicated that the NFκB pathway-associated genes were induced after a TNFα treatment. We have shown that primary astrocytes devoid of microglia can respond to a TNFα treatment with the re-expression of genes implicated in the glial cell development.
Luxembourg Centre for Systems Biomedicine (LCSB): Biomedical Data Science (Glaab Group) ; Luxembourg Centre for Systems Biomedicine (LCSB): Experimental Neurobiology (Balling Group)
Researchers ; Professionals ; Students
http://hdl.handle.net/10993/22485
10.1016/j.gdata.2015.11.005
http://www.sciencedirect.com/science/article/pii/S221359601530074X
FnR ; FNR5782168 > Enrico Glaab > ExPDIENT > Exploring Parkinson’s Disease Inhibitor Efficacy on a Non-dopaminergic Target > 01/12/2013 > 31/05/2016 > 2013

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