Reference : Temperature and nitric oxide control spontaneous calcium transients in astrocytes.
Scientific journals : Article
Life sciences : Multidisciplinary, general & others
http://hdl.handle.net/10993/22353
Temperature and nitric oxide control spontaneous calcium transients in astrocytes.
English
Schipke, Carola G. [> >]
Heidemann, Antje [> >]
Skupin, Alexander mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
Peters, Oliver [> >]
Falcke, Martin [> >]
Kettenmann, Helmut [> >]
2008
Cell calcium
43
3
285-95
Yes (verified by ORBilu)
International
0143-4160
Scotland
[en] Animals ; Astrocytes/cytology/drug effects/metabolism ; Calcium Signaling/drug effects ; Cells, Cultured ; Cerebral Cortex/cytology/drug effects/metabolism ; Mice ; Nitric Oxide/pharmacology ; Nitric Oxide Donors/pharmacology ; Perfusion ; S-Nitrosoglutathione/pharmacology ; Temperature ; Time Factors
[en] Transient spontaneous increases in the intracellular Ca2+ concentration have been frequently observed in astrocytes in cell culture and in acutely isolated slices from several brain regions. Recent in vivo experiments, however, reported only a low frequency of spontaneous Ca2+ events in astrocytes. Since the ex vivo experiments were usually performed at temperatures lower than physiological body temperature, we addressed the question whether temperature could influence the spontaneous Ca2+ activity in astrocytes. Indeed, comparing the frequency and spike width of spontaneous Ca2+ transients in astrocytes at temperatures between 20 and 37 degrees C in culture as well as in acute cortical slices from mouse brain, revealed that spontaneous Ca2+ responses occurred frequently at low temperature and became less frequent at higher temperature. Moreover, the single Ca2+ events had a longer duration at low temperature. We found that nitric oxide (NO) mimicked the increase in spontaneous Ca2+ activity and that an NO-synthase inhibitor attenuated the effect of lowering the temperature. Thus, temperature and NO are major determinants of spontaneous astrocytic Ca2+ signalling.
http://hdl.handle.net/10993/22353

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