Reference : In situ phenotypic heterogeneity among single cells of the filamentous bacterium Cand...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Environmental sciences & ecology
Life sciences : Microbiology
http://hdl.handle.net/10993/22293
In situ phenotypic heterogeneity among single cells of the filamentous bacterium Candidatus Microthrix parvicella
English
Sheik, Abdul mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Muller, Emilie mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Audinot, Jean-Nicolas []
Lebrun, Laura mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Grysan, Patrick []
Guignard, Cédric []
Wilmes, Paul mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
2015
ISME Journal (The)
Yes
International
1751-7362
1751-7370
[en] Microorganisms in biological wastewater treatment plants require adaptive strategies to deal with rapidly fluctuating environmental conditions. At the population level, the filamentous bacterium Candidatus Microthrix parvicella (Ca. M. parvicella) has been found to fine-tune its gene expression for optimized substrate assimilation. Here we investigated in situ substrate assimilation by single cells of Ca. M. parvicella using nano-scale secondary-ion mass spectrometry (nanoSIMS). NanoSIMS imaging highlighted phenotypic heterogeneity among Ca. M. parvicella cells of the same filament, whereby 13C-oleic acid and 13C-glycerol-3-phosphate assimilation occurred in ≈21–55% of cells, despite non-assimilating cells being intact and alive. In response to alternating aerobic–anoxic regimes, 13C-oleic acid assimilation occurred among subpopulations of Ca. M. parvicella cells (≈3–28% of cells). Furthermore, Ca. M. parvicella cells exhibited two temperature optima for 13C-oleic acid assimilation and associated growth rates. These results suggest that phenotypic heterogeneity among Ca. M. parvicella cells allows the population to adapt rapidly to fluctuating environmental conditions facilitating its widespread occurrence in biological wastewater treatment plants.
Luxembourg Centre for Systems Biomedicine (LCSB): Eco-Systems Biology (Wilmes Group)
http://hdl.handle.net/10993/22293
10.1038/ismej.2015.181

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
ismej2015181a.pdfPublisher postprint2.91 MBView/Open

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.