Reference : Dynamic regulation of microRNA expression following interferonγ- induced gene transcr...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/20247
Dynamic regulation of microRNA expression following interferonγ- induced gene transcription
English
Reinsbach, Susanne mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Nazarov, P. V. [Microarray Center, Centre de Recherche Public de la Santé, Luxembourg, Luxembourg]
Philippidou, Demetra mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Schmitt, Michèle mailto [University of Luxembourg > Central Administration > Student Department]
Wienecke-Baldacchino, Anke mailto [> >]
Muller, A. [> >]
Vallar, L. [> >]
Behrmann, Iris mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Kreis, Stephanie mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
2012
RNA Biology
9
7
987-989
Yes (verified by ORBilu)
International
1547-6286
1555-8584
[en] Dynamic expression; MicroRNA; Time-series microarrays; Transcriptional regulation
[en] MicroRNAs are major players in post-transcriptional gene regulation. Even small changes in miRNA levels may have profound consequences for the expression levels of target genes. Hence, miRNAs themselves need to be tightly, albeit dynamically, regulated. Here, we investigated the dynamic behavior of miRNAs over a wide time range following stimulation of melanoma cells with interferonγ (IFNγ), which activates the transcription factor STAT1. By applying several bioinformatic and statistical software tools for visualization and identification of differentially expressed miRNAs derived from time-series microarray experiments, 8.9% of 1105 miRNAs appeared to be directly or indirectly regulated by STAT1. Focusing on distinct dynamic expression patterns, we found that the majority of robust miRNA expression changes occurred in the intermediate time range (24-48 h). Three miRNAs (miR-27a, miR-30a and miR-34a) had a delayed regulation occurring at 72 h while none showed significant expression changes at early time points between 30 min and 6 h. Expression patterns of individual miRNAs were altered gradually over time or abruptly increased or decreased between two time points. Furthermore, we observed coordinated dynamic transcription of most miRNA clusters while few were found to be regulated independently of their genetic cluster. Most interestingly, several "star" or passenger strand sequences were specifically regulated over time while their "guide" strands were not. © 2012 Landes Bioscience.
http://hdl.handle.net/10993/20247
10.4161/rna.20494
http://www.scopus.com/inward/record.url?eid=2-s2.0-84865278613&partnerID=40&md5=b7eb9c38273f11f78547bb47194ecac6
cited By (since 1996)6
Scopus

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