Reference : An essential role of syntaxin 3 protein for granule exocytosis and secretion of IL-1α...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/19680
An essential role of syntaxin 3 protein for granule exocytosis and secretion of IL-1α, IL-1β, IL-12b, and CCL4 from differentiated HL-60 cells
English
Naegelen, Isabelle mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit > Calcium Signaling and Inflammation]
Plançon, Sébastien mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit > Calcium Signaling and Inflammation]
Nicot, Nathalie [Centre de Recherche Public Santé, Luxembourg]
Kaoma, Tony [Centre de Recherche Public Santé, Luxembourg]
Muller, Arnaud [Centre de Recherche Public Santé, Luxembourg]
Vallar, Laurent [Centre de Recherche Public Santé, Luxembourg]
Tschirhart, Eric mailto [University of Luxembourg > Central Administration > >]
Bréchard, Sabrina mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit > Calcium Signaling and Inflammation]
29-Dec-2014
Journal of Leukocyte Biology
Wiley Liss, Inc.
97
Yes (verified by ORBilu)
International
0741-5400
New York
NY
[en] SNARE proteins ; cytokines ; extracellular release ; gelatinase granules ; neutrophils
[en] Besides their roles in the killing of pathogens, neutrophils have the capacity to package a variety of cytokines into cytoplasmic granules for subsequent release upon inflammatory conditions. Because the rapid secretion of cytokines orchestrates the action of other immune cells at the infection site and thus, can contribute to the development and chronicity of inflammatory diseases, we aimed to determine the intracellular SNARE machinery responsible for the regulation of cytokine secretion and degranulation. From a constructed gene-expression network, we first selected relevant cytokines for functional validation by the CBA approach. We established a cytokine-secretion profile for human neutrophils and dHL-60 cells, underlining their similar ability to secrete a broad variety of cytokines within proinflammatory conditions mimicked by LPS stimulation. Secondly, after screening of SNARE genes by microarray experiments, we selected STX3 for further functional studies. With the use of a siRNA strategy, we show that STX3 is clearly required for the maximal release of IL-1α, IL-1β, IL-12b, and CCL4 without alteration of other cytokine secretion in dHL-60 cells. In addition, we demonstrate that STX3 is involved in MMP-9 exocytosis from gelatinase granules, where STX3 is partly localized. Our results suggest that the secretion of IL-1α, IL-1β, IL-12b, and CCL4 occurs during gelatinase degranulation, a process controlled by STX3. In summary, these findings provide first evidence that STX3 has an essential role in trafficking pathways of cytokines in neutrophil granulocytes.
http://hdl.handle.net/10993/19680
10.1189/jlb.3A0514-254RR

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