Reference : The sequential isolation of metabolites, RNA, DNA, and proteins from a single, undivi...
Scientific journals : Other
Life sciences : Environmental sciences & ecology
Life sciences : Microbiology
http://hdl.handle.net/10993/18994
The sequential isolation of metabolites, RNA, DNA, and proteins from a single, undivided mixed microbial community sample
English
Muller, Emilie mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Buschart, Anna mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Roume, Hugo [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Lebrun, Laura mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Wilmes, Paul mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
2014
Protocol Exchange
Nature Publishing Group
No
International
[en] Biomolecular isolation ; Eco-Systems Biology ; Integrated omics ; Microbial community ; Multi-omics
[en] Integrated omics of microbial consortia, comprising systematized metagenomic, metatranscriptomic, metaproteomic and meta-metabolomic analyses, allows in-depth characterization of organismal and functional diversity in situ. To allow meaningful meta-omic data integration, truly systematic measurements of the typically heterogeneous sample biomass is required. Therefore, there is a need for analyzing biomolecular fractions obtained from single, undivided samples. Here, we share a methodological workflow for the reproducible isolation of concomitant polar and non-polar metabolites, RNA, DNA and proteins from samples obtained from a biological wastewater treatment plant. The methodological framework is applicable to other biological samples [1,2], is compatible with different kits for biomacromolecular isolation [1,2] with minimal tailoring, and represents an important first step in standardization for the emerging field of Molecular Eco-Systems Biology.
Luxembourg Centre for Systems Biomedicine (LCSB): Eco-Systems Biology (Wilmes Group)
http://hdl.handle.net/10993/18994
10.1038/protex.2014.051

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