Reference : NTFD - A stand-alone application for the non-targeted detection of stable isotope lab...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/10993/1829
NTFD - A stand-alone application for the non-targeted detection of stable isotope labeled compounds in GC/MS data.
English
Hiller, Karsten mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Wegner, André mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Weindl, Daniel mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Cordes, Thekla mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Metallo, C. M. [> >]
Kelleher, J. K. [> >]
Stephanopoulos, G. [> >]
11-Mar-2013
Bioinformatics
Oxford University Press - Journals Department
29
9
1226-8
Yes (verified by ORBilu)
International
1367-4803
1460-2059
Oxford
United Kingdom
[en] NTFD ; isotope labeling ; non-targeted
[en] SUMMARY: Most current stable isotope-based methodologies are targeted and focus only on the well-described aspects of metabolic networks. Here, we present NTFD (non-targeted tracer fate detection), a software for the non-targeted analysis of all detectable compounds derived from a stable isotope-labeled tracer present in a GC/MS dataset. In contrast to traditional metabolic flux analysis approaches, NTFD does not depend on any a priori knowledge or library information. To obtain dynamic information on metabolic pathway activity, NTFD determines mass isotopomer distributions for all detected and labeled compounds. These data provide information on relative fluxes in a metabolic network. The graphical user interface allows users to import GC/MS data in netCDF format and export all information into a tab-separated format.
AVAILABILITY: NTFD is C++- and Qt4-based, and it is freely available under an open-source license. Pre-compiled packages for the installation on Debian- and Redhat-based Linux distributions, as well as Windows operating systems, along with example data, are provided for download at http://ntfd.mit.edu/.
CONTACT:
gregstep@mit.edu.
Luxembourg Centre for Systems Biomedicine (LCSB): Metabolomics (Hiller Group)
http://hdl.handle.net/10993/1829
10.1093/bioinformatics/btt119
http://bioinformatics.oxfordjournals.org/content/29/9/1226.long

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