Reference : Stereospecific modulation of the calcium channel in human erythrocytes by cholesterol...
Scientific journals : Article
Human health sciences : Multidisciplinary, general & others
http://hdl.handle.net/10993/18086
Stereospecific modulation of the calcium channel in human erythrocytes by cholesterol and its oxidized derivatives.
English
Neyses, Ludwig mailto [University of Luxembourg > Research Office]
Locher, R. [> >]
Stimpel, M. [> >]
Streuli, R. [> >]
Vetter, W. [> >]
1985
The Biochemical journal
227
1
105-12
Yes (verified by ORBilu)
International
0264-6021
ENGLAND
[en] Calcium/blood ; Calcium Channel Blockers/pharmacology ; Cholesterol/analogs & derivatives/pharmacology ; Erythrocytes/drug effects/metabolism ; Humans ; Ion Channels/drug effects/metabolism ; Kinetics ; Molecular Conformation ; Nifedipine/analogs & derivatives/pharmacology ; Nitrendipine
[en] To study the effect of cholesterol and its pathophysiologically important oxidized derivatives (OSC) on the calcium entry channel, the human red blood cell was used as a model system. The calcium ejecting adenosinetriphosphatase (ATPase) was inhibited by vanadate. The cells were loaded with OSC at concentrations between 1.25 X 10(-5) and 25 X 10(-5) mol/l. 22-Hydroxycholesterol, cholestan-3 beta,5 alpha,6 beta-triol, 5 alpha-cholestan-3 beta-ol,3 beta,5 alpha-dihydroxycholestan-6-one and 3 beta-hydroxy-5 alpha-cholestan-7-one stimulated 45Ca2+ influx by up to almost 90%, whereas 25-hydroxycholesterol, 7 beta-hydroxycholesterol, 20 alpha-hydroxycholesterol and 7-oxocholesterol inhibited influx by up to 75%. Both stimulation and inhibition were dependent on the amount of OSC incorporated into the membrane. More than 90% of the total modification of calcium influx by OSC was accounted for by an influence on the nitrendipine-inhibitable part of influx. Enrichment of cholesterol in the membrane greatly stimulated, and cholesterol depletion inhibited, Ca2+ influx. These results demonstrate that cholesterol and its oxidized derivatives are able to modulate the calcium channel in human red blood cells in a highly stereospecific manner.
http://hdl.handle.net/10993/18086
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1144814/

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