Reference : Chromosomal haplotypes by genetic phasing of human families
Scientific journals : Article
Life sciences : Multidisciplinary, general & others
http://hdl.handle.net/10993/16030
Chromosomal haplotypes by genetic phasing of human families
English
Roach, Jared C. [Institute for Systems Biology]
Glusman, Gustavo [> >]
Hubley, Robert [> >]
Montsaroff, Stephen Z. [> >]
Holloway, Alisha K. [> >]
Mauldin, Denise E. [> >]
Srivastava, Deepak [> >]
Garg, Vidu [> >]
Pollard, Katherine S. [> >]
Galas, David J. [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > > ; Institute for Systems Biology]
Hood, Leroy [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > > ; Institute for Systems Biology]
Smit, Arian F. A. [> >]
18-Aug-2011
American Journal of Human Genetics
Cell Press
89
3
382-397
Yes (verified by ORBilu)
0002-9297
1537-6605
[en] Assignment of alleles to haplotypes for nearly all the variants on all chromosomes can be performed by genetic analysis of a nuclear family with three or more children. Whole-genome sequence data enable deterministic phasing of nearly all sequenced alleles by permitting assignment of recombinations to precise chromosomal positions and specific meioses. We demonstrate this process of genetic phasing on two families each with four children. We generate haplotypes for all of the children and their parents; these haplotypes span all genotyped positions, including rare variants. Misassignments of phase between variants (switch errors) are nearly absent. Our algorithm can also produce multimegabase haplotypes for nuclear families with just two children and can handle families with missing individuals. We implement our algorithm in a suite of software scripts (Haploscribe). Haplotypes and family genome sequences will become increasingly important for personalized medicine and for fundamental biology.
Luxembourg Centre for Systems Biomedicine (LCSB): Experimental Neurobiology (Balling Group)
http://hdl.handle.net/10993/16030
10.1016/j.ajhg.2011.07.023

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