Reference : Janus kinases as targets for small molecule inhibitors
Dissertations and theses : Doctoral thesis
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/15687
Janus kinases as targets for small molecule inhibitors
English
Kaczor, Jakub [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
5-Jul-2010
University of Luxembourg, ​Luxembourg, ​​Luxembourg
Docteur en Biologie
Behrmann, Iris mailto
[en] Janus kinase Erlotinib ; Interferon signaling Jak2-V617F ; Analogue-sensitive
[en] Many cytokines transduce signals by employing receptor/Janus kinase (Jak) complexes that, once activated, promote phosphorylation of several signaling proteins such as STAT transcription factors. The Jak/STAT pathway is normally tightly modulated by regulatory mechanisms preventing its over-activation. However, a number of genetic alterations in Jak kinase genes have been found in myeloproliferative neoplasms and leukemia that render the Jaks hyperactive and cytokine-independent. In the present thesis pharmacologic and genetic approaches were applied to study aspects of both the cytokine-dependent and -independent activation of the Jak/STAT pathway.
We applied a chemical genetics approach to Janus kinases and generate analogue-sensitive Jak1 and Jak2 mutants. With these tools we could for the first time specifically inhibit either Jak1 or Jak2 and investigate their relative contributions in IFN signaling. Single inhibition of either Jak by an inhibitor analogue showed differential effects on IFN-mediated STAT1 phosphorylation, Jak2 inhibition having a stronger suppressive effect. Furthermore, we identified STAT1 and IRF1 as genes, whose increased expression upon IFN stimulation strongly depends on active Jak2 but not on Jak1. Most of the IFN-regulated genes, however, depend on both Jak1 and Jak2 activity. Exploiting this quick and reversible pharmacological inhibition of analogue-sensitive kinases we address the temporal requirement of Jak activity and the importance of different phases of the IFN response.
Moreover, we further characterized constitutively active Jak2 mutants and show that SOCS proteins seem to be directly involved in their proteasome-mediated degradation. There is an increasing interest in developing small molecule inhibitors targeting Jaks and Jak mutants. We show that Erlotinib, an inhibitor targeting EGFR which is already used in the clinic to treat lung and pancreatic cancer, can have a beneficial additional effect if combined with a Jak-specific inhibitor on cell growth suppression and apoptosis of Jak2-V617F- or Jak3-A572V-positive leukemic cells. Erlotinib, in contrast to Jak inhibitors targeting Jaks directly affects the eIF2/ATF4 pathway in leukemic cells. This is of general interest since Erlotinib treatment in conjunction with other kinase inhibitors could be an interesting approach also in other kinase-dependant leukemias (e.g. BCR-ABL-positive CML).
http://hdl.handle.net/10993/15687

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